PMID- 8522323
OWN - NLM
STAT- MEDLINE
DCOM- 19960122
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 96
IP  - 6
DP  - 1995 Dec
TI  - Complete and precise characterization of marker chromosomes by application of 
      microdissection in prenatal diagnosis.
PG  - 661-7
AB  - A straightforward and extremely efficient reverse chromosome painting technique 
      is described which allows the rapid and unequivocal identification of any 
      cytogenetically unclassifiable chromosome rearrangement. This procedure is used 
      to determine the origin of unknown marker chromosomes found at prenatal 
      diagnosis. After microdissection of the marker chromosome and amplification of 
      the dissected fragment by a degenerate oligonucleotide-primed polymerase chain 
      reaction (DOP-PCR), fluorescence in situ hybridization (FISH) to aberrant and 
      normal metaphase chromosomes with the marker-derived probe pool is performed. 
      With this strategy, marker chromosomes present in amniotic fluid samples were 
      successfully identified in three cases. The origin of the supernumerary markers 
      was ascertained as deriving from 3p(p12-cen), 18p(pter-cen) and 9p(p12-cen), 
      respectively. Since a specific FISH signal on chromosomes can be obtained within 
      2 working days using a probe generated without any pretreatment from one 
      chromosomal fragment only and without additional image processing devices, this 
      technique is considered to be highly suitable for routine application in pre- and 
      postnatal cytogenetic analysis.
FAU - Muller-Navia, J
AU  - Muller-Navia J
AD  - Institut fur Anthropologie, Universitat Mainz, Germany.
FAU - Nebel, A
AU  - Nebel A
FAU - Schleiermacher, E
AU  - Schleiermacher E
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - *Amniocentesis
MH  - Amniotic Fluid/cytology
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, Pair 3
MH  - *Chromosomes, Human, Pair 9
MH  - DNA Primers
MH  - Dissection/methods
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infant, Newborn
MH  - Karyotyping
MH  - Maternal Age
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - Pregnancy, High-Risk
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1007/BF00210295 [doi]
PST - ppublish
SO  - Hum Genet. 1995 Dec;96(6):661-7. doi: 10.1007/BF00210295.