PMID- 8522521
OWN - NLM
STAT- MEDLINE
DCOM- 19960122
LR  - 20191210
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 177
IP  - 24
DP  - 1995 Dec
TI  - Expression and characterization of the Escherichia coli fdo locus and a possible 
      physiological role for aerobic formate dehydrogenase.
PG  - 7141-9
AB  - In the presence of nitrate, the major anaerobic respiratory pathway includes 
      formate dehydrogenase (FDH-N) and nitrate reductase (NAR-A), which catalyze 
      formate oxidation coupled to nitrate reduction. Two aerobically expressed 
      isoenzymes, FDH-Z and NAR-Z, have been recently characterized. Enzymatic analysis 
      of plasmid subclones carrying min 88 of the Escherichia coli chromosome was 
      consistent with the location of the fdo locus encoding FDH-Z between the fdhD and 
      fdhE genes which are necessary for the formation of both formate dehydrogenases. 
      The fdo locus produced three proteins (107, 34, and 22 kDa) with sizes similar to 
      those of the subunits of the purified FDH-N. In support to their structural role, 
      these polypeptides were recognized by antibodies specific to FDH-N. Expression of 
      a chromosomal fdo-uidA operon fusion was induced threefold by aerobic growth and 
      about twofold by anaerobic growth in the presence of nitrate. However, it was 
      independent of the two global regulatory proteins FNR and ArcA, which control 
      genes for anaerobic and aerobic functions, respectively, and of the nitrate 
      response regulator protein NARL. In contrast, a mutation affecting either the 
      nucleoid-associated H-NS protein or the CRP protein abolished the aerobic 
      expression. A possible role for FDH-Z during the transition from aerobic to 
      anaerobic conditions was examined. Synthesis of FDH-Z was maximal at the end of 
      the aerobic growth and remained stable after a shift to anaerobiosis, whereas 
      FDH-N production developed only under anaerobiosis. Furthermore, in an fnr strain 
      deprived of both FDH-N and NAR-A activities, aerobically expressed FDH-Z and 
      NAR-Z enzymes were shown to reduce nitrate at the expense of formate under 
      anaerobic conditions, suggesting that this pathway would allow the cell to 
      respond quickly to anaerobiosis.
FAU - Abaibou, H
AU  - Abaibou H
AD  - Laboratoire de Genetique Moleculaire des Microorganismes et des Interactions 
      Cellulaires, Institut National des Sciences Appliquees, Centre National de la 
      Recherche Scientifique URA 1486, Villeurbanne, France.
FAU - Pommier, J
AU  - Pommier J
FAU - Benoit, S
AU  - Benoit S
FAU - Giordano, G
AU  - Giordano G
FAU - Mandrand-Berthelot, M A
AU  - Mandrand-Berthelot MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - EC 1.17.1.9 (Formate Dehydrogenases)
SB  - IM
MH  - Aerobiosis
MH  - Cloning, Molecular
MH  - Escherichia coli/*enzymology/genetics
MH  - Formate Dehydrogenases/*genetics/metabolism
MH  - *Gene Expression Regulation, Bacterial
MH  - *Gene Expression Regulation, Enzymologic
MH  - Plasmids/genetics
PMC - PMC177593
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
PMCR- 1995/12/01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PHST- 1995/12/01 00:00 [pmc-release]
AID - 10.1128/jb.177.24.7141-7149.1995 [doi]
PST - ppublish
SO  - J Bacteriol. 1995 Dec;177(24):7141-9. doi: 10.1128/jb.177.24.7141-7149.1995.