PMID- 8522613
OWN - NLM
STAT- MEDLINE
DCOM- 19960123
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 131
IP  - 6 Pt 1
DP  - 1995 Dec
TI  - Hepatocyte growth factor/scatter factor induces a variety of tissue-specific 
      morphogenic programs in epithelial cells.
PG  - 1573-86
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is the mesenchymal ligand of the 
      epithelial tyrosine kinase receptor c-Met. In vitro, HGF/SF has morphogenic 
      properties, e.g., induces kidney epithelial cells to form branching ducts in 
      collagen gels. Mutation of the HGF/SF gene in mice results in embryonic lethality 
      due to severe liver and placenta defects. Here, we have evaluated the morphogenic 
      activity of HGF/SF with a large variety of epithelial cells grown in 
      three-dimensional collagen matrices. We found that HGF/SF induces SW 1222 colon 
      carcinoma cells to form crypt-like structures. In these organoids, cells exhibit 
      apical/basolateral polarity and build a well-developed brush border towards the 
      lumen. Capan 2 pancreas carcinoma cells, upon addition of HGF/SF, develop large 
      hollow spheroids lined with a tight layer of polarized cells. Collagen inside the 
      cysts is digested and the cells show features of pancreatic ducts. HGF/SF induces 
      EpH4 mammary epithelial cells to form long branches with end-buds that resemble 
      developing mammary ducts. pRNS-1-1 prostate epithelial cells in the presence of 
      HGF/SF develop long ducts with distal branching as found in the prostate. 
      Finally, HGF/SF simulates alveolar differentiation in LX-1 lung carcinoma cells. 
      Expression of transfected HGF/SF cDNA in LX-1 lung carcinoma and EpH4 mammary 
      epithelial cells induce morphogenesis in an autocrine manner. In the cell lines 
      tested, HGF/SF activated the Met receptor by phosphorylation of tyrosine 
      residues. These data show that HGF/SF induces intrinsic, tissue-specific 
      morphogenic activities in a wide variety of epithelial cells. Apparently, HGF/SF 
      triggers respective endogenous programs and is thus an inductive, not an 
      instructive, mesenchymal effector for epithelial morphogenesis.
FAU - Brinkmann, V
AU  - Brinkmann V
AD  - Max-Delbruck-Center for Molecular Medicine, Berlin, Germany.
FAU - Foroutan, H
AU  - Foroutan H
FAU - Sachs, M
AU  - Sachs M
FAU - Weidner, K M
AU  - Weidner KM
FAU - Birchmeier, W
AU  - Birchmeier W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adenocarcinoma
MH  - Animals
MH  - Carcinoma
MH  - Cell Size/physiology
MH  - Colonic Neoplasms
MH  - Dogs
MH  - Embryonic and Fetal Development/physiology
MH  - Epithelial Cells
MH  - Epithelium/physiology/ultrastructure
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Kidney/cytology
MH  - Liver/*cytology/*growth & development
MH  - Lung/cytology
MH  - Male
MH  - Mice
MH  - Microscopy, Electron
MH  - Morphogenesis/physiology
MH  - Pancreas/cytology
MH  - Phosphorylation
MH  - Prostate/cytology
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Tumor Cells, Cultured/cytology/physiology/ultrastructure
PMC - PMC2120656
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
PMCR- 1996/06/02
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PHST- 1996/06/02 00:00 [pmc-release]
AID - 96101935 [pii]
AID - 10.1083/jcb.131.6.1573 [doi]
PST - ppublish
SO  - J Cell Biol. 1995 Dec;131(6 Pt 1):1573-86. doi: 10.1083/jcb.131.6.1573.