PMID- 8524266
OWN - NLM
STAT- MEDLINE
DCOM- 19960119
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 15
IP  - 12
DP  - 1995 Dec
TI  - Constitutive repression and nuclear factor I-dependent hormone activation of the 
      mouse mammary tumor virus promoter in Saccharomyces cerevisiae.
PG  - 6987-98
AB  - To study the influence of various transactivators and the role of nucleosomal 
      structure in gene regulation by steroid hormones, we have introduced mouse 
      mammary tumor virus (MMTV) promoter sequences along with expression vectors for 
      the glucocorticoid receptor (GR) and nuclear factor I (NFI) in Saccharomyces 
      cerevisiae, an organism amenable to genetic manipulation. Both in the context of 
      an episomal multicopy vector and in a centromeric single-copy plasmid, the MMTV 
      promoter was virtually silent in the absence of inducer, even in yeast strains 
      expressing GR and NFI. Induction was optimal with deacylcortivazol and required 
      both GR and NFI. The transactivation function AF1 in the N-terminal half of GR is 
      required for ligand-dependent induction and acts constitutively in truncated GR 
      lacking the ligand binding domain. A piece of the MMTV long terminal repeat 
      extending from -236 to +111 is sufficient to position a nucleosome, B, over the 
      regulatory region of the promoter from -45 to -190 and another nucleosome over 
      the transcription start region. The rotational orientation of the DNA on the 
      surface of nucleosome B is the same as that previously found in animal cells and 
      in reconstitution experiments. This orientation is compatible with binding of GR 
      to two sites, while it should preclude binding of NFI and hence be responsible 
      for constitutive repression. Upon ligand induction, there is no major chromatin 
      rearrangement, but the proximal linker DNA, including the TATA box, becomes 
      hypersensitive to nucleases. The transcriptional behavior of the MMTV promoter 
      was unaffected by deletions of the genes for zuotin or SIN1/SPT2, two proteins 
      which have been claimed to assume some of the functions of linker histones. Thus, 
      despite the lack of histone H1, yeast cells could be a suitable system to study 
      the contribution of nucleosomal organization to the regulated expression of the 
      MMTV promoter.
FAU - Chavez, S
AU  - Chavez S
AD  - Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat, Marburg, 
      Germany.
FAU - Candau, R
AU  - Candau R
FAU - Truss, M
AU  - Truss M
FAU - Beato, M
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (Pregnatrienes)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
RN  - 3JO09QT49F (deacylcortivazol)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - DNA Footprinting
MH  - DNA Primers
MH  - DNA-Binding Proteins/biosynthesis/*metabolism
MH  - Genetic Vectors
MH  - Genotype
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Nucleosomes/metabolism
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Pregnatrienes/*pharmacology
MH  - *Promoter Regions, Genetic/drug effects
MH  - Receptors, Glucocorticoid/biosynthesis/drug effects/*metabolism
MH  - Recombinant Proteins/biosynthesis/metabolism
MH  - Repetitive Sequences, Nucleic Acid
MH  - Saccharomyces cerevisiae/genetics/*metabolism
MH  - TATA Box
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/biosynthesis/metabolism
MH  - Transcription, Genetic/drug effects/*physiology
MH  - Y-Box-Binding Protein 1
MH  - beta-Galactosidase/biosynthesis
PMC - PMC230954
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
PMCR- 1995/12/01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
PHST- 1995/12/01 00:00 [pmc-release]
AID - 10.1128/MCB.15.12.6987 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1995 Dec;15(12):6987-98. doi: 10.1128/MCB.15.12.6987.