PMID- 8524364
OWN - NLM
STAT- MEDLINE
DCOM- 19960123
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 348
IP  - 3
DP  - 1995 Nov
TI  - Comparison of gamma-ray induced dicentric yields in human lymphocytes measured by 
      conventional analysis and FISH.
PG  - 125-30
AB  - In an earlier work stable aberrations and dicentrics were determined by 
      fluorescence in situ hybridization (FISH) after various doses of 137Cs 
      gamma-rays. No corresponding calibration curve for dicentrics is available for 
      determinations in terms of the conventional analysis as performed in our 
      laboratory. In view of the potential for the application of chromosome painting 
      to human biological dosimetry, it is desirable to determine such a calibration 
      curve and this and the comparison of the resulting data to those obtained in 
      terms of the FISH method is the objective of the present communication. In the 
      study it is found that the linear-quadratic dose response curves for dicentrics, 
      that are determined by the two methods, are significantly different, although the 
      different target sizes are accounted for. A similar problem was found earlier for 
      X-rays. It does not appear that the difference is due to technical difficulties 
      in the FISH method, that has been improved by employing in addition to the whole 
      chromosome DNA probes, a pan-centromeric DNA probe.
FAU - Schmid, E
AU  - Schmid E
AD  - Institut fur Strahlenbiologie, GSF-Forschungszentrum, Oberschleissheim, Germany.
FAU - Braselmann, H
AU  - Braselmann H
FAU - Nahrstedt, U
AU  - Nahrstedt U
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Cesium Isotopes)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Cell Cycle/genetics
MH  - Cesium Isotopes
MH  - Chromosome Aberrations
MH  - DNA Probes
MH  - Dose-Response Relationship, Radiation
MH  - *Gamma Rays
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*radiation effects/ultrastructure
MH  - Metaphase/genetics
MH  - Mutagenicity Tests/*methods
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 0165-7992(95)00056-9 [pii]
AID - 10.1016/0165-7992(95)00056-9 [doi]
PST - ppublish
SO  - Mutat Res. 1995 Nov;348(3):125-30. doi: 10.1016/0165-7992(95)00056-9.