PMID- 8524640
OWN - NLM
STAT- MEDLINE
DCOM- 19960125
LR  - 20191210
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 23
IP  - 22
DP  - 1995 Nov 25
TI  - The transcription factors ATF-1 and CREB-1 bind constitutively to the 
      hypoxia-inducible factor-1 (HIF-1) DNA recognition site.
PG  - 4542-50
AB  - The hypoxia-inducible factor-1 (HIF-1) was first described as a DNA binding 
      activity that specifically recognizes an 8 bp motif known to be essential for 
      hypoxia-inducible erythropoietin gene transcription. Subsequently HIF-1 activity 
      has also been found in cell lines which do not express erythropoietin, suggesting 
      that HIF-1 is part of a widespread oxygen sensing mechanism. In electrophoretic 
      mobility shift assays HIF-1 DNA binding activity is only detectable in nuclear 
      extracts of cells cultivated in a low oxygen atmosphere. In addition to HIF-1, a 
      constitutive DNA binding activity also specifically binds the HIF1 probe. Here we 
      report that CRE and AP1 oligonucleotides efficiently competed for binding of the 
      HIF1 probe to this constitutive factor, whereas HIF-1 activity itself remained 
      unaffected. Monoclonal antibodies raised against the CRE binding factors ATF-1 
      and CREB-1 supershifted the constitutive factors ATF-1 and CREB-1 supershifted 
      the constitutive factor, while Jun and Fos family members, which constitute the 
      AP-1 factor, were immunologically undetectable. Recombinant ATF-1 and CREB-1 
      proteins bound HIF1 probes either as homodimers or as heterodimers, indicating a 
      new binding specificity for ATF-1/CREB-1. Finally, reporter gene assays in HeLa 
      cells treated with either a cAMP analogue or a phorbol ester suggest that the 
      PKA, but not the PKC signalling pathway is involved in oxygen sensing.
FAU - Kvietikova, I
AU  - Kvietikova I
AD  - Physiologisches Institut, Universitat Zurich-Irchel, Switzerland.
FAU - Wenger, R H
AU  - Wenger RH
FAU - Marti, H H
AU  - Marti HH
FAU - Gassmann, M
AU  - Gassmann M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Activating Transcription Factor 1)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Atf1 protein, mouse)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Activating Transcription Factor 1
MH  - Activating Transcription Factor 2
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Nucleus/metabolism
MH  - Consensus Sequence
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - DNA/*chemistry/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - L Cells
MH  - Leucine Zippers
MH  - Luciferases/analysis/biosynthesis
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*metabolism
MH  - Oligodeoxyribonucleotides
MH  - Recombinant Proteins/biosynthesis/metabolism
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription Factors/isolation & purification/*metabolism
MH  - Transfection
PMC - PMC307423
EDAT- 1995/11/25 00:00
MHDA- 1995/11/25 00:01
CRDT- 1995/11/25 00:00
PHST- 1995/11/25 00:00 [pubmed]
PHST- 1995/11/25 00:01 [medline]
PHST- 1995/11/25 00:00 [entrez]
AID - 5w0172 [pii]
AID - 10.1093/nar/23.22.4542 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1995 Nov 25;23(22):4542-50. doi: 10.1093/nar/23.22.4542.