PMID- 8527398
OWN - NLM
STAT- MEDLINE
DCOM- 19960126
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 14
IP  - 2
DP  - 1995 Oct
TI  - Isochromosome 17q demonstrated by interphase fluorescence in situ hybridization 
      in primitive neuroectodermal tumors of the central nervous system.
PG  - 85-96
AB  - We previously reported an i(17q) as a non-random finding in childhood primitive 
      neuroectodermal tumors (PNETs) of the central nervous system. In the present 
      study, we describe a two-color interphase fluorescence in situ hybridization 
      (FISH) assay for detection of chromosome 17 abnormalities in tumors. Thirty-four 
      PNETs were analyzed by FISH with a series of chromosome 17-specific probes which 
      map to 17p13.3-17q25. The results from the FISH assay were then compared to the 
      karyotypes prepared from the tumors. Ten of the 34 cases demonstrated an i(17q) 
      by FISH and standard cytogenetics. Two PNETs were shown to have an i(17q) by FISH 
      alone, and three additional tumors had deletions of 17p. Thus, a total of 15 of 
      34 (44%) of the PNETs in this series had a deletion of 17p. This study confirms 
      and extends our previous reports that an i(17q) is the most common cytogenetic 
      abnormality in PNETs. The interphase FISH assay which we employed will have 
      clinical utility for diagnosis of children with malignant brain tumors, and it 
      may be used for identification of tumors with 17p deletions for molecular studies 
      aimed at identifying disease genes.
FAU - Biegel, J A
AU  - Biegel JA
AD  - Division of Human Genetics and Molecular Biology, Children's Hospital of 
      Philadelphia, PA 19104, USA.
FAU - Rorke, L B
AU  - Rorke LB
FAU - Janss, A J
AU  - Janss AJ
FAU - Sutton, L N
AU  - Sutton LN
FAU - Parmiter, A H
AU  - Parmiter AH
LA  - eng
GR  - CA13539/CA/NCI NIH HHS/United States
GR  - CA46274/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Central Nervous System Neoplasms/*genetics/pathology
MH  - Child
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 17
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - *Isochromosomes
MH  - Karyotyping
MH  - Male
MH  - Neuroectodermal Tumors, Primitive/*genetics/pathology
MH  - Sequence Deletion
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1002/gcc.2870140202 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1995 Oct;14(2):85-96. doi: 10.1002/gcc.2870140202.