PMID- 8527798
OWN - NLM
STAT- MEDLINE
DCOM- 19960130
LR  - 20061115
IS  - 0916-8478 (Print)
IS  - 0916-8478 (Linking)
VI  - 40
IP  - 3
DP  - 1995 Sep
TI  - Cot-1 banding of human chromosomes using fluorescence in situ hybridization with 
      Cy3 labeling.
PG  - 243-52
AB  - We developed a new chromosome banding method by in situ hybridization of human 
      Cot-1 DNA as a probe. Clear banding was produced on metaphase chromosomes of 
      lymphoblastoid cells after probe detection with a fluorescent dye Cy3. Comparison 
      with the known banding patterns revealed a similarity to the R-banding with some 
      significant differences: some centromeric heterochromatin regions show Cot-1 
      positive bands. This suggests that some repetitive sequences from the 
      heterochromatin regions constitute a major component of Cot-1 DNA. This unique 
      chromosome banding method, Cot-1 banding, may be used as a supplement to the 
      conventional karyotype analysis. Scanning analysis of the fluorescence 
      intensities of Cot-1 banding and Q-banding are useful for objectively analyzing 
      the banding pattern including a detection of chromosome aberrations. The Cot-1 
      banding with Cy3 is particularly powerful when applied for the gene mapping by 
      fluorescence in situ hybridization (FISH) because red fluorescence of Cy3 for 
      chromosome staining can be readily distinguished from green fluorescence of 
      fluorescein isothiocyanate (FITC) for probe labeling. Using this novel method, we 
      mapped a 4 kb-DNA fragment from myelin protein zero (MPZ) gene on the chromosome 
      1q22 to q23.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of Molecular Biology, Keio University School of Medicine, Tokyo, 
      Japan.
FAU - Minoshima, S
AU  - Minoshima S
FAU - Shimizu, N
AU  - Shimizu N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Hum Genet
JT  - The Japanese journal of human genetics
JID - 9213239
RN  - 0 (Carbocyanines)
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Myelin P0 Protein)
RN  - 0 (cyanine dye 3)
SB  - IM
MH  - *Carbocyanines
MH  - Chromosome Banding/*methods
MH  - Chromosomes, Human, Pair 1
MH  - DNA Probes
MH  - *Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Myelin P0 Protein/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1007/BF01876182 [doi]
PST - ppublish
SO  - Jpn J Hum Genet. 1995 Sep;40(3):243-52. doi: 10.1007/BF01876182.