PMID- 8528585
OWN - NLM
STAT- MEDLINE
DCOM- 19960201
LR  - 20191101
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 7
DP  - 1995 Nov
TI  - Confirmation of the copy number of chromosome 1 in interphase nuclei from 
      paraffin sections of breast tumours by fluorescence in situ hybridization.
PG  - 410-6
AB  - Fluorescence in situ hybridization (FISH) was used to establish the copy number 
      of chromosome 1 in a set of nine breast tumours in which the chromosome had 
      previously been shown to have undergone a variety of rearrangements by loss of 
      heterozygosity studies. In each case, FISH with satellite III DNA from chromosome 
      1q12 confirmed the results obtained by Southern hybridization. Importantly, in 
      all five cases with rearrangements thought not to involve the centromeric region, 
      FISH showed that the events had not disrupted the gross chromosome structure. 
      This study highlights the potential of using the two techniques together to 
      obtain a clearer picture of both large- and small-scale alterations to 
      chromosomes in solid tumours.
FAU - Loupart, M L
AU  - Loupart ML
AD  - ICI/University Joint Laboratory, University of Leicster, UK.
FAU - Walker, R
AU  - Walker R
FAU - Brammar, W
AU  - Brammar W
FAU - Varley, J
AU  - Varley J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Alleles
MH  - Blotting, Southern
MH  - Breast Neoplasms/*genetics
MH  - Centromere/genetics
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - DNA Probes
MH  - DNA, Satellite/genetics
MH  - Female
MH  - Heterozygote
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Paraffin Embedding
MH  - Trisomy/genetics
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1007/BF00713890 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Nov;3(7):410-6. doi: 10.1007/BF00713890.