PMID- 8528680
OWN - NLM
STAT- MEDLINE
DCOM- 19960201
LR  - 20181130
IS  - 1018-7782 (Print)
IS  - 1018-7782 (Linking)
VI  - 3
IP  - 6
DP  - 1995 Nov-Dec
TI  - Physiological and biochemical response of glomerular epithelial cells to 
      exogenous epidermal growth factor.
PG  - 355-61
AB  - Epidermal growth factor (EGF) is a growth-promoting cytokine which acts in a 
      paracrine and autocrine fashion on epithelial cells of various tissues. Although 
      previously demonstrated, we have now confirmed the presence of EGF receptors in 
      cultured glomerular epithelial cells (GEC) using radioligand binding studies. 
      Further, the biochemical consequences of EGF receptor activation in this cell 
      type were investigated. In the presence of exogenous EGF, attachment of GEC to 
      plastic was enhanced in a dose-dependent (0.62-10 ng/ml range) manner. At 10 
      ng/ml of EGF, cell attachment was 5 times higher than in controls. After 
      attachment, there was a 3-fold increase in cell proliferation rate in the 
      presence of 10 ng/ml of EGF. Cells which multiplied and reached confluency in the 
      presence of EGF were 80% larger in volume than in controls. In the presence of 
      EGF, a 70% maximal increase in de novo protein synthetic activity as tested by 
      3H-leucine incorporation was observed in the dose range of 0.62-10 ng/ml. 
      Further, a dose-dependent increase in extracellular type IV collagenase 
      (gelatinase) as well as in intracellular cathepsins B and D (150 and 260% of 
      control, respectively) activities were noted. It is concluded that GEC respond to 
      exogenous EGF by increasing their protein synthetic rate as well as their 
      catabolic rate, thus resulting in enhanced turnover of macromolecules.
FAU - Singh, A K
AU  - Singh AK
AD  - Department of Medicine, Loyola-Hines Medical Center, Maywood, Ill., USA.
FAU - Song, R H
AU  - Song RH
FAU - Frankfater, A F
AU  - Frankfater AF
FAU - Leehey, D J
AU  - Leehey DJ
LA  - eng
GR  - DK35804/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Exp Nephrol
JT  - Experimental nephrology
JID - 9302239
RN  - 0 (Plastics)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.23.5 (Cathepsin D)
RN  - EC 3.4.24.- (Gelatinases)
SB  - IM
MH  - Animals
MH  - Cathepsin B/metabolism
MH  - Cathepsin D/metabolism
MH  - Cell Adhesion
MH  - Cell Division
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/*pharmacology
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - ErbB Receptors/*metabolism
MH  - Gelatinases/metabolism
MH  - Kidney Glomerulus/cytology/*metabolism
MH  - Mice
MH  - Plastics
MH  - Protein Biosynthesis
MH  - Rats
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - Exp Nephrol. 1995 Nov-Dec;3(6):355-61.