PMID- 8530448
OWN - NLM
STAT- MEDLINE
DCOM- 19960130
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 51
DP  - 1995 Dec 22
TI  - Urokinase-type plasminogen activator-induced monocyte adhesion requires a 
      carboxyl-terminal lysine and cAMP-dependent signal transduction.
PG  - 30282-5
AB  - Urokinase-type plasminogen activator (u-PA) or its amino-terminal fragment (ATF) 
      containing the u-PA receptor (u-PAR) binding domain, is known to promote monocyte 
      adhesion. In the present study, U937 monocyte adhesion to a plastic surface was 
      used to investigate the mechanism of its promotion by u-PA and ATF. Adhesion was 
      found to be inhibited by cycloheximide or actinomycin D, implicating protein 
      synthesis and gene expression in u-PA-induced monocyte adhesion. Adhesion was 
      prevented by 2'-deoxyadenosine 3'-monophosphate, indicating that a cAMP-dependent 
      pathway of signal transduction was involved. This concept was supported by the 
      complementary finding that u-PA-induced adhesion was greatly promoted by 
      forskolin, cholera toxin, or 8-bromo-cAMP, which by themselves induced little 
      adhesion. Furthermore, similar to many other cAMP-dependent activities, cGMP 
      diminished u-PA-induced adhesion. When u-PA or ATF was treated with immobilized 
      carboxypeptidase B, its proadhesive effect was abolished, implicating the 
      involvement of carboxyl-terminal lysine residues (Lys158 on u-PA and Lys135 on 
      ATF). Moreover, when a carboxyl-terminal lysine analog was added, the proadhesive 
      effect of carboxypeptidase B-treated u-PA or ATF was restored. In conclusion, the 
      present study indicates that u-PA- or ATF-induced monocyte adhesion involves 
      cAMP-dependent signal transduction, which is triggered by u-PAR binding. It is 
      also critically dependent on the presence of a carboxyl-terminal lysine.
FAU - Li, C
AU  - Li C
AD  - Vascular Research Laboratory, Deaconess Hospital, Harvard Medical School, Boston, 
      Massachusetts 02215, USA.
FAU - Liu, J N
AU  - Liu JN
FAU - Gurewich, V
AU  - Gurewich V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (PLAUR protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 12UHW9R67N (Isoflurophate)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 1F7A44V6OU (Colforsin)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 31356-94-2 (8-bromocyclic GMP)
RN  - 98600C0908 (Cycloheximide)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Binding Sites
MH  - Cell Adhesion/drug effects/*physiology
MH  - Cell Line
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*metabolism
MH  - Cyclic GMP/analogs & derivatives/pharmacology
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Humans
MH  - Isoflurophate/pharmacology
MH  - Kinetics
MH  - Monocytes/drug effects/*physiology
MH  - Peptide Fragments/pharmacology
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Urokinase-Type Plasminogen Activator/*pharmacology
EDAT- 1995/12/22 00:00
MHDA- 1995/12/22 00:01
CRDT- 1995/12/22 00:00
PHST- 1995/12/22 00:00 [pubmed]
PHST- 1995/12/22 00:01 [medline]
PHST- 1995/12/22 00:00 [entrez]
AID - S0021-9258(17)31128-6 [pii]
AID - 10.1074/jbc.270.51.30282 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Dec 22;270(51):30282-5. doi: 10.1074/jbc.270.51.30282.