PMID- 8534702
OWN - NLM
STAT- MEDLINE
DCOM- 19960202
LR  - 20061115
IS  - 1197-8554 (Print)
IS  - 1197-8554 (Linking)
VI  - 11
DP  - 1995
TI  - MCP-1 levels are elevated in peritonitis fluid from CAPD patients due to 
      secretion by peritoneal macrophages.
PG  - 19-23
AB  - The migration of leukocytes, including polymorphonuclear neutrophils and 
      monocytes, into the peritoneal cavity is a key event of intraperitoneal 
      inflammation. We investigated the levels of two members of the chemokine family, 
      interleukin 8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1), in the 
      dialysate effluent of 18 continuous ambulatory peritoneal dialysis (CAPD) 
      patients with peritonitis and compared them with chemokine levels in noninfected 
      CAPD effluent. Being a major source of inflammatory cytokines, we also isolated 
      peritoneal macrophages from peritonitis effluent to determine the mRNA expression 
      directly after isolation. The mean (SEM) concentrations of IL-8 and MCP-1 were 
      significantly higher in the effluent of peritonitis patients than in noninfected 
      effluents MCP-1: 22.5 +/- (6.27) versus 0.37 +/- (0.1) ng/mL and IL-8: 2.39 +/- 
      (1.15) versus 0.04 +/- (0.01) ng/mL. Northern blot analysis of isolated effluent 
      macrophages revealed strong signals for MCP-1 and IL-8. Our findings showed that 
      CAPD effluent from patients with peritonitis contains markedly elevated MCP-1 and 
      IL-8 levels, suggesting that these chemokines participate in leukocyte 
      recruitment during CAPD peritonitis. Isolation of mRNA of peritonitis-derived 
      peritoneal macrophages revealed strong signals for MCP-1 and IL-8, suggesting 
      that macrophages are a major source of these inflammatory mediators.
FAU - Sach, M
AU  - Sach M
AD  - Department of Nephrology, Freiburg University Hospital, Germany.
FAU - Loetscher, P
AU  - Loetscher P
FAU - Burger, J A
AU  - Burger JA
FAU - Knopf, H P
AU  - Knopf HP
FAU - Schollmeyer, P
AU  - Schollmeyer P
FAU - Dobos, G J
AU  - Dobos GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Adv Perit Dial
JT  - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
JID - 9104803
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dialysis Solutions)
RN  - 0 (Interleukin-8)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Ascitic Fluid/*chemistry
MH  - Blotting, Northern
MH  - Chemokine CCL2/*metabolism
MH  - Dialysis Solutions/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Macrophages, Peritoneal/*metabolism
MH  - *Peritoneal Dialysis, Continuous Ambulatory/adverse effects
MH  - Peritonitis/etiology/*metabolism
MH  - RNA/analysis
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Adv Perit Dial. 1995;11:19-23.