PMID- 8535071
OWN - NLM
STAT- MEDLINE
DCOM- 19960208
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 6
IP  - 9
DP  - 1995 Sep
TI  - Chromosomal localization and molecular characterization of 53 cosmid-derived 
      bovine microsatellites.
PG  - 629-35
AB  - Gene mapping in cattle has progressed rapidly in recent years largely owing to 
      the introduction of powerful genetic markers, such as the microsatellites, and 
      through advances in physical mapping techniques such as synteny mapping and 
      fluorescence in situ hybridization (FISH). Microsatellite markers are often not 
      physically mapped because they are generally isolated from small insert plasmid 
      libraries, which makes their chromosomal localization inefficient. In this report 
      we describe the FISH mapping of a large group of cosmid-derived bovine 
      microsatellite markers, as our contribution to the European mapping initiative, 
      BovMap. One objective of BovMap is to develop a set of anchored loci for the 
      cattle genome map. Two cosmid libraries were screened with probes corresponding 
      to the (AC)n microsatellite motif. Positive clones were mapped by FISH, and then 
      a subset was further analyzed by sequencing the region flanking the 
      microsatellite repeat. In total, 58 clones were hybridized with chromosomes and 
      identified loci on 22 of the 31 different bovine chromosomes. Three clones 
      contained satellite DNA. Two or more markers were placed on 12 chromosomes. 
      Sequencing of the microsatellites and flanking regions was performed directly 
      from 43 cosmids, as previously reported (Ferretti et al. Anim. Genet. 25, 
      209-214, 1994). Primers were developed for 39 markers and used to describe the 
      polymorphism associated with the corresponding loci.
FAU - Mezzelani, A
AU  - Mezzelani A
AD  - Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Milano, 
      Italy.
FAU - Zhang, Y
AU  - Zhang Y
FAU - Redaelli, L
AU  - Redaelli L
FAU - Castiglioni, B
AU  - Castiglioni B
FAU - Leone, P
AU  - Leone P
FAU - Williams, J L
AU  - Williams JL
FAU - Toldo, S S
AU  - Toldo SS
FAU - Wigger, G
AU  - Wigger G
FAU - Fries, R
AU  - Fries R
FAU - Ferretti, L
AU  - Ferretti L
LA  - eng
SI  - GENBANK/X85045
SI  - GENBANK/X85046
SI  - GENBANK/X85047
SI  - GENBANK/X85048
SI  - GENBANK/X85049
SI  - GENBANK/X85050
SI  - GENBANK/X85051
SI  - GENBANK/X85053
SI  - GENBANK/X85054
SI  - GENBANK/X85055
SI  - GENBANK/X85056
SI  - GENBANK/X85057
SI  - GENBANK/X85058
SI  - GENBANK/X85059
SI  - GENBANK/X85060
SI  - GENBANK/X85061
SI  - GENBANK/X85062
SI  - GENBANK/X85063
SI  - GENBANK/X85064
SI  - GENBANK/X85065
SI  - GENBANK/X85066
SI  - GENBANK/X85067
SI  - GENBANK/X85068
SI  - GENBANK/X85069
SI  - GENBANK/X85070
SI  - GENBANK/X85071
SI  - GENBANK/X85072
SI  - GENBANK/X85073
SI  - GENBANK/X85074
SI  - GENBANK/X85075
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - *Chromosome Mapping
MH  - Cosmids/genetics
MH  - DNA Primers
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1007/BF00352370 [doi]
PST - ppublish
SO  - Mamm Genome. 1995 Sep;6(9):629-35. doi: 10.1007/BF00352370.