PMID- 8536234
OWN - NLM
STAT- MEDLINE
DCOM- 19960208
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 85
IP  - 1
DP  - 1995 Nov
TI  - Correlation of loss of heterozygosity with cytogenetic analysis using G-banding 
      and fluorescence in situ hybridization in aneuploid cultures from two human 
      testicular germ-cell tumors.
PG  - 26-36
AB  - A detailed karyotype analysis using fluorescence in situ hybridization (FISH), 
      with 24 chromosome-specific paint probes has been carried out on newly 
      established cell lines from two testicular tumors, an i(12p)-positive teratoma, 
      and an i(12p)-negative combined seminoma/teratoma. This has been correlated with 
      loss of heterozygosity (LOH) and allelic imbalance, using DNA RFLP analysis to 
      clarify the genetic changes and to identify any common regions of deletion or 
      rearrangement. With G-banding alone, a total of 11 breakpoints were recognized. 
      After FISH, the position of seven required revision, and 21 new ones were 
      identified. The chromosomes involved most frequently in both tumors were numbers 
      1, 12, and 18. Breakpoints in 11q and 16q were also seen in both, and seven or 
      more copies of 12p per cell were found in all clones. LOH was found for 18q in 
      both tumors, and overall was much more frequent in underrepresented regions (one 
      or two copies). On the whole, there was good agreement between the cytogenetic 
      and DNA RFLP data; loci showing allelic imbalance generally had an odd number of 
      copies of the chromosome region in which they were known to be located. Combined 
      data on the chromosome 1 translocations in both tumors suggested that 
      rearrangements were more complicated than cytogenetics alone had predicted.
FAU - al-Jehani, R M
AU  - al-Jehani RM
AD  - Galton Laboratory, Department of Genetics and Biometry, University College 
      London, UK.
FAU - Wells, D
AU  - Wells D
FAU - Fox, M
AU  - Fox M
FAU - Parrington, J M
AU  - Parrington JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Alleles
MH  - *Aneuploidy
MH  - Blotting, Southern
MH  - Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Gene Deletion
MH  - *Heterozygote
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Polymorphism, Restriction Fragment Length
MH  - Seminoma/*genetics
MH  - Teratoma/*genetics
MH  - Testicular Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 0165460895001190 [pii]
AID - 10.1016/0165-4608(95)00119-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Nov;85(1):26-36. doi: 10.1016/0165-4608(95)00119-0.