PMID- 8536235
OWN - NLM
STAT- MEDLINE
DCOM- 19960208
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 85
IP  - 1
DP  - 1995 Nov
TI  - Single-cell trisomy in hematologic malignancy. Random change or tip of the 
      iceberg?
PG  - 37-42
AB  - Finding a clone in the bone marrow of a patient with a hematologic disorder is 
      important to confirm the neoplastic nature of the disease and may be indicative 
      of prognosis. Since cytogenetic analysis detects only actively dividing clones, 
      the presence of a single abnormal cell among 20 cells analyzed raises doubts 
      about its clonal nature. Fluorescence in situ hybridization (FISH) enables rapid 
      detection of certain chromosomal abnormalities in metaphase and interphase cells, 
      thus enabling detection of minor or inactive clones. Seven patients with 
      hematologic malignancy each having random cell(s) were investigated thus: at 
      diagnosis, with MDS and a cell with +8 (two cases) or +9 (one case) and with AML 
      and a cell with +4 (one case), +7 (one case), or two cells with +9, +22/ +10, 
      +17, +17 (one case). One patient with ALL in remission had one cell with trisomy 
      4. One patient, a male aged 66 years with refractory anemia with ringed 
      sideroblasts, was found to have a minor trisomy 8 clone in his diagnostic marrow. 
      A follow-up marrow 42 months later showed no trisomy 8 cell among 62 metaphases 
      analyzed, and the percentage of trisomic cells using FISH on interphase cells was 
      within the control range. This patient has survived for more than 42 months 
      requiring no treatment. Single-cell abnormalities in the other six cases proved 
      to be random events. Thus it appears that single-cell abnormalities may not be 
      clonal or at most indicate the presence of a minor clone well below the level of 
      cytogenetic detection. The prognostic significance of such minor clones is at 
      present unclear.
FAU - Kasprzyk, A
AU  - Kasprzyk A
AD  - Department of Haematology, Royal Free Hospital School of Medicine, London, UK.
FAU - Mehta, A B
AU  - Mehta AB
FAU - Secker-Walker, L M
AU  - Secker-Walker LM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
CIN - Cancer Genet Cytogenet. 1996 Aug;90(1):91-2. PMID: 8780756
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology/ultrastructure
MH  - Child
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Myelodysplastic Syndromes/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prognosis
MH  - *Trisomy
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 0165460895001158 [pii]
AID - 10.1016/0165-4608(95)00115-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Nov;85(1):37-42. doi: 10.1016/0165-4608(95)00115-8.