PMID- 8541531
OWN - NLM
STAT- MEDLINE
DCOM- 19960213
LR  - 20210322
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 86
IP  - 12
DP  - 1995 Dec 15
TI  - A human monoclonal antibody specific for the leucine-33 (P1A1, HPA-1a) form of 
      platelet glycoprotein IIIa from a V gene phage display library.
PG  - 4430-6
AB  - IgG alloantibodies to polymorphic platelet glycoproteins (GPs) are known to be 
      responsible for severe thrombocytopenia in the neonate and after transfusion. 
      Platelet GPIIIa can have either a leucine or a proline at residue 33. The most 
      immunogenic platelet alloantigen in thrombocytopenia is the leucine 33 form of 
      GPIIIa. Here, we have generated human monoclonal antibody fragments that are 
      specific for the leucine and not the proline form of GPIIIa and can inhibit the 
      binding of polyclonal human IgG alloantibodies to GPIIIa leucine 33 on platelets. 
      The antibody fragments were selected from a library of single chain Fv fragments 
      displayed on the surface of filamentous phage. The VH gene repertoire was derived 
      from the peripheral blood lymphocytes of an alloimmunized individual and 
      recombined with a VL gene repertoire from a nonimmune source. Antibodies such as 
      these, which are able to distinguish between two variant forms of a native 
      antigen and which have been unobtainable by conventional hybridoma technology, 
      have both diagnostic and potential therapeutic applications.
FAU - Griffin, H M
AU  - Griffin HM
AD  - Division of Transfusion Medicine, University of Cambridge, UK.
FAU - Ouwehand, W H
AU  - Ouwehand WH
LA  - eng
SI  - GENBANK/S80752
SI  - GENBANK/S80758
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (ITGB3 protein, human)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Integrin beta3)
RN  - 0 (Isoantibodies)
RN  - 0 (Peptide Fragments)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Recombinant Fusion Proteins)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/genetics/*immunology/isolation & purification
MH  - Antibody Specificity
MH  - Antigens, CD/*immunology
MH  - Antigens, Human Platelet/*immunology
MH  - Base Sequence
MH  - *Coliphages/genetics
MH  - Female
MH  - *Genes, Immunoglobulin
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Immunization
MH  - Immunoglobulin G/genetics/immunology
MH  - Immunoglobulin M/genetics/immunology
MH  - Immunoglobulin Variable Region/*genetics
MH  - Integrin beta3
MH  - Isoantibodies/genetics/*immunology/isolation & purification
MH  - Leucine
MH  - Lymphocytes/chemistry
MH  - Molecular Sequence Data
MH  - Peptide Fragments/*immunology
MH  - Phenotype
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/*immunology
MH  - Pregnancy
MH  - Recombinant Fusion Proteins/genetics/*immunology/isolation & purification
MH  - Sequence Alignment
MH  - Sequence Homology
EDAT- 1995/12/15 00:00
MHDA- 1995/12/15 00:01
CRDT- 1995/12/15 00:00
PHST- 1995/12/15 00:00 [pubmed]
PHST- 1995/12/15 00:01 [medline]
PHST- 1995/12/15 00:00 [entrez]
AID - S0006-4971(20)65044-3 [pii]
PST - ppublish
SO  - Blood. 1995 Dec 15;86(12):4430-6.