PMID- 8543372
OWN - NLM
STAT- MEDLINE
DCOM- 19960213
LR  - 20190920
IS  - 0360-3997 (Print)
IS  - 0360-3997 (Linking)
VI  - 19
IP  - 5
DP  - 1995 Oct
TI  - Cytokine-induced neutrophil chemoattractant production by primary rat alveolar 
      type II cells.
PG  - 575-86
AB  - This study was designed to determine the production of the chemokine 
      cytokine-induced neutrophil chemoattractant (CINC) by primary rat alveolar type 
      II (ATII) cells upon stimulation with exogenous and endogenous proinflammatory 
      factors. Cultures of primary rat ATII cells were exposed to lipopolysaccharide 
      (LPS), interleukin-1 beta (IL-1 beta) or tumor necrosis factor-alpha (TNF alpha) 
      over a 16 hour period and the production of CINC both apically and basolaterally 
      was measured by ELISA. Compared to unstimulated (UNS) cultures, LPS, IL-1 beta 
      and TNF alpha were found to significantly increase the level of CINC detected in 
      culture by two, four and sixteen hours post stimulation, respectively. ATII cells 
      also demonstrated a polar secretion of CINC. The accumulation of CINC 
      basolaterally was significantly more than apically; 133%, 45%, 117% and 123% for 
      UNS, IL-1 beta, LPS and TNF alpha respectively. We demonstrated that primary rat 
      ATII cells may participate in the chemokine network during inflammation by the 
      production of CINC upon stimulation with endogenous and exogenous factors.
FAU - Crippen, T L
AU  - Crippen TL
AD  - Department of Anatomy, Physiology and Cell Biology, University of California, 
      Davis 95616, USA.
FAU - Klasing, K C
AU  - Klasing KC
FAU - Hyde, D M
AU  - Hyde DM
LA  - eng
GR  - P01 ES-00626-18/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Growth Substances/*biosynthesis
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Pulmonary Alveoli/cytology/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1007/BF01539137 [doi]
PST - ppublish
SO  - Inflammation. 1995 Oct;19(5):575-86. doi: 10.1007/BF01539137.