PMID- 8544404
OWN - NLM
STAT- MEDLINE
DCOM- 19960214
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 48
IP  - 5
DP  - 1995 Nov
TI  - Production and cytokine-mediated regulation of monocyte chemoattractant protein-1 
      by human proximal tubular epithelial cells.
PG  - 1477-86
AB  - Impairment of renal function in various types of glomerular disease is associated 
      with tubulointerstitial changes. The mechanism of mononuclear cell infiltration 
      in the interstitium is not fully understood. Recently, monocyte chemoattractant 
      protein-1 (MCP-1) has been identified as a monocyte-specific chemotactic factor. 
      We analyzed the presence of MCP-1 in renal biopsies from patients with various 
      forms of glomerular disease and demonstrated that MCP-1 expression is increased 
      in renal tubular epithelial cells during disease. Further analysis showed that 
      various cell lines of human proximal tubular epithelial cells (PTEC) produce 
      MCP-1 in culture under serum-free conditions and that the production is inhibited 
      by cycloheximide. IL-1 alpha and TNF-alpha enhanced the production by each cell 
      line in a dose- and time-dependent manner as measured by radioimmunoassay. 
      Northern blot analysis demonstrated that IL-1 alpha and TNF-alpha markedly 
      enhanced the expression of MCP-1 mRNA. Taken together these observations support 
      the notion that MCP-1 is synthesized de novo by PTEC. MCP-1 produced by PTEC is 
      found to be 13 kD by gel filtration chromatography. It is chemotactically active 
      for monocytes. We conclude that in various types of glomerular disease, MCP-1 
      expression in tubular epithelial cells is associated with up-regulation of MCP-1 
      production by PTEC. These findings raise the possibility that macrophages may 
      accumulate in renal interstitium as a consequence of MCP-1 production by PTEC.
FAU - Prodjosudjadi, W
AU  - Prodjosudjadi W
AD  - Department of Nephrology, University Hospital Leiden, The Netherlands.
FAU - Gerritsma, J S
AU  - Gerritsma JS
FAU - Klar-Mohamad, N
AU  - Klar-Mohamad N
FAU - Gerritsen, A F
AU  - Gerritsen AF
FAU - Bruijn, J A
AU  - Bruijn JA
FAU - Daha, M R
AU  - Daha MR
FAU - van Es, L A
AU  - van Es LA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Biopsy
MH  - Blotting, Northern
MH  - Chemokine CCL2/*metabolism/physiology
MH  - Chemotaxis, Leukocyte/physiology
MH  - Cytokines/*physiology
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Kidney/metabolism/pathology
MH  - Kidney Tubules, Proximal/cytology/*metabolism
MH  - Monocytes/physiology
MH  - Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - S0085-2538(15)59209-0 [pii]
AID - 10.1038/ki.1995.437 [doi]
PST - ppublish
SO  - Kidney Int. 1995 Nov;48(5):1477-86. doi: 10.1038/ki.1995.437.