PMID- 8546705
OWN - NLM
STAT- MEDLINE
DCOM- 19960214
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 313 ( Pt 1)
IP  - Pt 1
DP  - 1996 Jan 1
TI  - Structural characterization of the latent complex between transforming growth 
      factor beta 1 and beta 1-latency-associated peptide.
PG  - 343-51
AB  - The formation of a non-covalent complex between mature transforming growth factor 
      beta 1 (TGF-beta 1) and its pro region, the beta 1-latency-associated peptide 
      (beta 1-LAP), is important in regulating the activity of this multipotent growth 
      factor. We have overexpressed simian beta 1-LAP in Chinese hamster ovary (CHO) 
      cells to produce a cell line which secretes beta 1-LAP into the culture medium at 
      > 1 mg/l, thus enabling structural studies of complex formation between beta 
      1-LAP and TGF-beta 1. The simian beta 1-LAP expressed in CHO cells reversed the 
      growth inhibitory effect of exogenous TGF-beta 1 on Mv1Lu (mink lung epithelial) 
      cells and was able to form a cross-linked complex with 125I-TGF-beta 1. Simian 
      beta 1-LAP was purified to homogeneity by a combination of ammonium sulphate 
      precipitation, gel filtration, dye ligand chromatography and anion-exchange 
      chromatography, with a yield of 15%. The purified protein had an apparent 
      molecular mass of 114 kDa as determined by SDS/PAGE, which is greater than that 
      determined for the transient expression of simian beta 1-LAP in COS-1 and for the 
      simian precursor of TGF-beta 1 (pro-TGF-beta 1) in CHO cells, this major 
      difference being due to more extensive glycosylation of beta 1-LAP expressed by 
      this CHO clone. Far-UV CD spectroscopy of simian beta 1-LAP indicates a mostly 
      beta-sheet structure, with extensive structural rearrangements occurring upon 
      formation of the latent complex between TGF-beta 1 and beta 1-LAP.
FAU - McMahon, G A
AU  - McMahon GA
AD  - Biochemistry and Molecular Biology, Medical College of Ohio, Toledo 43699-0008, 
      USA.
FAU - Dignam, J D
AU  - Dignam JD
FAU - Gentry, L E
AU  - Gentry LE
LA  - eng
GR  - CA60848/CA/NCI NIH HHS/United States
GR  - DE09669/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Carbohydrates)
RN  - 0 (DNA, Complementary)
RN  - 0 (Mannosephosphates)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Precursors)
RN  - 0 (Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 3672-15-9 (mannose-6-phosphate)
RN  - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
SB  - IM
MH  - Animals
MH  - CHO Cells/metabolism
MH  - Carbohydrates/analysis
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Chemical Precipitation
MH  - Chromatography, Ion Exchange
MH  - Circular Dichroism
MH  - Cricetinae
MH  - DNA, Complementary/genetics
MH  - Gene Amplification
MH  - Lung/cytology/drug effects
MH  - Mannosephosphates/analysis
MH  - Mink
MH  - *Peptide Fragments
MH  - *Protein Precursors
MH  - Protein Structure, Secondary
MH  - Proteins/*isolation & purification/*metabolism/physiology
MH  - Tetrahydrofolate Dehydrogenase/genetics
MH  - Transforming Growth Factor beta/chemistry/*metabolism/pharmacology
MH  - Transforming Growth Factor beta1
PMC - PMC1216904
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
PMCR- 1996/07/01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PHST- 1996/07/01 00:00 [pmc-release]
AID - 10.1042/bj3130343 [doi]
PST - ppublish
SO  - Biochem J. 1996 Jan 1;313 ( Pt 1)(Pt 1):343-51. doi: 10.1042/bj3130343.