PMID- 8547133
OWN - NLM
STAT- MEDLINE
DCOM- 19960220
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 91
IP  - 4
DP  - 1995 Dec
TI  - Combined cytogenetic, FISH and molecular analysis in acute promyelocytic 
      leukaemia at diagnosis and in complete remission.
PG  - 878-84
AB  - This study reports the results of a simultaneous application of cytogenetic 
      fluorescence in situ hybridization (FISH) and molecular analysis (RT-PCR) in 28 
      APL cases (23 M3 and five M3v; 26 studied at diagnosis and two at relapse). FISH 
      on metaphases identified the t(15;17) in all cases who were positive for the 
      PML/RAR alpha transcript by RT-PCR. Conventional cytogenetics revealed the 
      t(15;17) in only 68% of cases. However, it enabled the detection of additional 
      chromosome changes in five cases, three of whom were M3v. 11 patients were also 
      investigated during complete remission (CR) by both FISH and RT-PCR, in order to 
      evaluate residual disease; the duration of CR at the time of analysis ranged 
      between 1 and 16 months, with three patients being studied twice. Comparison of 
      RT-PCR and FISH results showed a very good correlation. In fact, of the 10 
      samples which were RT-PCR positive for residual disease, all were also recognized 
      by interphase FISH, and eight were positive by metaphase FISH. Of the three 
      samples negative at RT-PCR, all were also negative at the interphase FISH. The 
      results of this study indicate that: (a) the t(15;17) is present in all cases 
      positive for the PML/RAR alpha rearrangement, thus in virtually all true APLs; 
      (b) standard cytogenetics, capable of unravelling the t(15;17) in only 68% of 
      cases, enables recognition of additional chromosome changes of potential clinical 
      and prognostic significance; (c) FISH on interphase nuclei is a reliable tool for 
      the monitoring of residual disease, with a sensitivity greater than that of FISH 
      on metaphase cells and superimposable to that of RT-PCR.
FAU - Mancini, M
AU  - Mancini M
AD  - Department of Human Biopathology, University of Rome La Sapienza, Italy.
FAU - Nanni, M
AU  - Nanni M
FAU - Cedrone, M
AU  - Cedrone M
FAU - Diverio, D
AU  - Diverio D
FAU - Avvisati, G
AU  - Avvisati G
FAU - Riccioni, R
AU  - Riccioni R
FAU - De Cuia, M R
AU  - De Cuia MR
FAU - Fenu, S
AU  - Fenu S
FAU - Alimena, G
AU  - Alimena G
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*diagnosis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual
MH  - Polymerase Chain Reaction
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - *Translocation, Genetic
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1995.tb05404.x [doi]
PST - ppublish
SO  - Br J Haematol. 1995 Dec;91(4):878-84. doi: 10.1111/j.1365-2141.1995.tb05404.x.