PMID- 8547654
OWN - NLM
STAT- MEDLINE
DCOM- 19960216
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 87
IP  - 1
DP  - 1996 Jan 1
TI  - Tumor-specific rearrangements of the immunoglobulin heavy-chain gene in B-cell 
      non-Hodgkin's lymphoma detected by in situ hybridization.
PG  - 292-8
AB  - We have recently described the potential use of fluorescence in situ 
      hybridization (FISH) to detect tumor-specific rearrangements of the 
      immunoglobulin heavy-chain (IgH) gene in interphase nuclei. Using yeast 
      artificial chromosome (YAC) clone Y6 containing variable region (VH) gene and 
      bacteriophage clones Ig gamma, we analyzed 70 patients with B-cell non-Hodgkin's 
      lymphoma (NHL) and compared the results with those obtained by the conventional 
      G-banding method. Tumor-specific rearrangements of the IgH gene equivalent to 
      14q32 translocations were defined as separate signals of VH and Ig gamma genes or 
      those of Ig gamma genes and referred to split signals. Twenty-nine patients 
      (41.4%) showed split signals. Among these, 13 did not show 14q32 translocations 
      by G-banding: three with other chromosomal abnormalities, one with normal 
      karyotype, and nine with no analyzable metaphases. The partner sites of 14q32 
      translocations were identified in 17 patients by FISH: t(3;14)(q27;q32) including 
      a complex variant was observed in nine patients, t(14;18)(q32;q21) in four, 
      t(8;14)(q24;q32) in three, t(14;19)(q32;q13) in one, and t(11;14)(q13;q32) in 
      one. Six of nine patients with t(3;14) or its variant and one of three with 
      t(8;14) were diagnosed as having respective translocations only by FISH. 
      Translocation t(3;14) was found most commonly, and was correlated histologically 
      with diffuse lymphoma with large-cell components. These results indicate that 
      interphase FISH with IgH gene probes promises to be a rapid and reliable method 
      for use in the diagnosis of B-cell NHL.
FAU - Ueda, Y
AU  - Ueda Y
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Matsuda, F
AU  - Matsuda F
FAU - Misawa, S
AU  - Misawa S
FAU - Taniwaki, M
AU  - Taniwaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 14/*ultrastructure
MH  - Chromosomes, Human, Pair 3/ultrastructure
MH  - DNA, Neoplasm/*genetics
MH  - Europe
MH  - Female
MH  - *Gene Rearrangement, B-Lymphocyte, Heavy Chain
MH  - *Genes, Immunoglobulin
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Japan
MH  - Lymphoma, B-Cell/*genetics
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Translocation, Genetic
MH  - United States
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - S0006-4971(20)66219-X [pii]
PST - ppublish
SO  - Blood. 1996 Jan 1;87(1):292-8.