PMID- 8548121
OWN - NLM
STAT- MEDLINE
DCOM- 19960221
LR  - 20190920
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 427
IP  - 4
DP  - 1995
TI  - Trisomy 1 and 8 occur frequently in hepatocellular carcinoma but not in liver 
      cell adenoma and focal nodular hyperplasia. A fluorescence in situ hybridization 
      study.
PG  - 373-8
AB  - Conventional cytogenetic studies revealed gains and structural aberrations of 
      chromosome 1 to be the most consistent chromosomal aberrations in hepatocellular 
      carcinoma (HCC). We investigated touch preparations of eight HCC, five 
      cholangiocellular carcinomas (CCC), five liver cell adenomas (LCA), four focal 
      nodular hyperplasias (FNH) as well as nine specimens of normal liver tissue using 
      fluorescence in situ hybridization (FISH) with centromere specific probes for 
      chromosomes 1 and 8. Polysomies of chromosome 1, especially trisomy 1, were found 
      in five of eight HCC and four of five CCC but in no normal liver tissue or benign 
      tumour. Only three of seven cases of HCC revealed trisomy 8 whereas the five 
      benign liver tumours and all normal liver tissues examined had disomy 8. Our 
      results confirm conventional cytogenetic findings in terms of chromosome 1 
      aberrations in HCC although they are not specific for these types of malignant 
      liver tumours. Since alpha-satellite probes were used in our study, only gains or 
      losses including the centromeric regions of the chromosomes 1 and 8 could be 
      detected. Nevertheless, our findings suggest that FISH may help in the 
      differential diagnosis of malignant versus benign neoplasms of the liver.
FAU - Nasarek, A
AU  - Nasarek A
AD  - Pathologisches Institut, Medizinische Hochschule Hannover, Germany.
FAU - Werner, M
AU  - Werner M
FAU - Nolte, M
AU  - Nolte M
FAU - Klempnauer, J
AU  - Klempnauer J
FAU - Georgii, A
AU  - Georgii A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
SB  - IM
MH  - Adenoma, Liver Cell/genetics
MH  - Carcinoma, Hepatocellular/*genetics
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - Hyperplasia/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Liver/pathology
MH  - Liver Neoplasms/*genetics
MH  - *Trisomy
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF00199385 [doi]
PST - ppublish
SO  - Virchows Arch. 1995;427(4):373-8. doi: 10.1007/BF00199385.