PMID- 8548731
OWN - NLM
STAT- MEDLINE
DCOM- 19960222
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 85
IP  - 2
DP  - 1995 Dec
TI  - Loss of chromosomes 22 and 14 in the malignant progression of meningiomas. A 
      comparative study of fluorescence in situ hybridization (FISH) and standard 
      cytogenetic analysis.
PG  - 101-4
AB  - The majority of meningiomas are classified as typical and have a relatively 
      benign course. However, approximately 10% are diagnosed as atypical, anaplastic, 
      or malignant and have a worse prognosis. The genetic differences between the 
      typical and higher grade meningiomas are not well characterized, although there 
      appear to be increasingly complex karyotypic changes associated with the higher 
      grade tumors. Because higher grade meningiomas are not common tumors, and because 
      of the inherent problems associated with the culturing of tumors, the use of 
      interphase cytogenetic techniques with paraffin-embedded archival material is 
      desirable for studying these neoplasms. To determine its accuracy in detecting 
      aneuploidy, we performed fluorescence in situ hybridization (FISH) on 2-micron 
      paraffin sections of nine previously karyotyped meningiomas using an 
      alpha-satellite probe for chromosomes 14 and 22. Sections of normal tissue from 
      six patients without malignancy were used as controls. FISH analysis detected all 
      of the chromosome losses in the meningioma cases that had been characterized 
      cytogenetically. In five cases, cell lines not detected by standard cytogenetics 
      were identified by FISH. These results indicate that FISH is a reliable method 
      for detecting chromosomal loss and may be more sensitive than standard 
      cytogenetics alone. Furthermore, the results of this study support the concept 
      that loss of chromosome 14 is associated with malignant progression in 
      meningiomas.
FAU - Schneider, B F
AU  - Schneider BF
AD  - Department of Pediatrics, University of Virginia School of Medicine, 
      Charlottesville, USA.
FAU - Shashi, V
AU  - Shashi V
FAU - von Kap-herr, C
AU  - von Kap-herr C
FAU - Golden, W L
AU  - Golden WL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Brain Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosome Deletion
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 22
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Meningioma/*genetics/pathology
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 0165460895001549 [pii]
AID - 10.1016/0165-4608(95)00154-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Dec;85(2):101-4. doi: 10.1016/0165-4608(95)00154-9.