PMID- 8548798
OWN - NLM
STAT- MEDLINE
DCOM- 19960221
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 83
IP  - 7
DP  - 1995 Dec 29
TI  - Reversal of intrinsic DNA bends in the IFN beta gene enhancer by transcription 
      factors and the architectural protein HMG I(Y).
PG  - 1101-11
AB  - In this paper, we investigate DNA bending induced by proteins required for virus 
      induction of the human interferon-beta (IFN beta) gene. We show that NF-kappa 
      B-DNA complexes that are functionally distinct in the context of the IFN beta 
      enhancer are also conformationally distinct and that two sites in the enhancer 
      contain in-phase bends that are counteracted or reversed by the binding of 
      NF-kappa B, ATF-2/c-Jun, and HMG I(Y). Strikingly, this modulation of intrinsic 
      enhancer architecture results in an orientation that favors predicted 
      protein-protein interactions in a functional nucleoprotein complex, the 
      enhanceosome. Furthermore, the subtle modulation of DNA structure by HMG I(Y) in 
      this process distinguishes it from other architectural factors.
FAU - Falvo, J V
AU  - Falvo JV
AD  - Department of Molecular and Cellular Biology, Harvard University, Cambridge, 
      Massachusetts 02138, USA.
FAU - Thanos, D
AU  - Thanos D
FAU - Maniatis, T
AU  - Maniatis T
LA  - eng
GR  - AI 20642/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Circular)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Transcription Factors)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Activating Transcription Factor 2
MH  - Base Sequence
MH  - Cyclic AMP Response Element-Binding Protein/genetics
MH  - DNA Probes
MH  - DNA, Circular/analysis/ultrastructure
MH  - Enhancer Elements, Genetic/*genetics
MH  - Gene Expression Regulation, Viral/genetics
MH  - HeLa Cells/physiology
MH  - High Mobility Group Proteins/*genetics
MH  - Humans
MH  - Interferon-beta/*genetics
MH  - Leucine Zippers/genetics
MH  - Molecular Sequence Data
MH  - NF-kappa B/genetics
MH  - Nucleic Acid Conformation
MH  - Proto-Oncogene Proteins c-jun/genetics
MH  - Transcription Factors/*genetics
MH  - Y Chromosome/*genetics
EDAT- 1995/12/29 00:00
MHDA- 1995/12/29 00:01
CRDT- 1995/12/29 00:00
PHST- 1995/12/29 00:00 [pubmed]
PHST- 1995/12/29 00:01 [medline]
PHST- 1995/12/29 00:00 [entrez]
AID - 0092-8674(95)90137-X [pii]
AID - 10.1016/0092-8674(95)90137-x [doi]
PST - ppublish
SO  - Cell. 1995 Dec 29;83(7):1101-11. doi: 10.1016/0092-8674(95)90137-x.