PMID- 8550891
OWN - NLM
STAT- MEDLINE
DCOM- 19960221
LR  - 20061115
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 361
IP  - 3
DP  - 1995 Oct 23
TI  - "Satellite cells" and nerve terminals in the crayfish opener muscle visualized 
      with fluorescent dyes.
PG  - 441-50
AB  - Nerve terminals and associated cells on the muscle's surface were visualized in 
      the crayfish opener muscle with several fluorescent dyes in conjunction with 
      confocal microscopy and conventional fluorescence microscopy. The nerve terminals 
      of the excitatory and inhibitory axons were best seen with 
      4-diethylaminostyryl-N-methylpyridinium iodide (4-Di-2-Asp). This dye is 
      selectively accumulated in mitochondria, which are numerous both in the axons and 
      in synapse-bearing terminal varicosities. Muscle nuclei were also clearly 
      visualized, because they excluded 4-Di-2-Asp but were stained by acridine orange 
      (AO). A positive attraction between muscle nuclei and nerve terminals was evident 
      by visual inspection and was confirmed by spatial statistics. Additional flat 
      cells on the muscle's surface appeared as bright rings with elongated processes 
      that were often close to or overlapped nearby nerve terminals. The structure of 
      these cells was established by electron microscopy after labeling them with 
      fluorescent polystyrene beads, which could be found over structures on the muscle 
      surface in sections of embedded specimens. The flat surface cells were distinct 
      from peripheral glial cells closely associated with axons and nerve terminals. 
      Nevertheless, spatial statistics showed that the surface cells were grouped near 
      nerve terminals. They occupied a small fraction of the muscle cell's surface. 
      Their functional role has not been determined in crustacean muscles.
FAU - Harrington, C C
AU  - Harrington CC
AD  - Department of Physiology, University of Toronto, Ontario, Canada.
FAU - Atwood, H L
AU  - Atwood HL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Astacoidea
MH  - *Fluorescent Dyes
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Muscles/*ultrastructure
MH  - Nerve Fibers/*ultrastructure
MH  - Neuromuscular Junction/ultrastructure
MH  - Presynaptic Terminals/*ultrastructure
EDAT- 1995/10/23 00:00
MHDA- 1995/10/23 00:01
CRDT- 1995/10/23 00:00
PHST- 1995/10/23 00:00 [pubmed]
PHST- 1995/10/23 00:01 [medline]
PHST- 1995/10/23 00:00 [entrez]
AID - 10.1002/cne.903610308 [doi]
PST - ppublish
SO  - J Comp Neurol. 1995 Oct 23;361(3):441-50. doi: 10.1002/cne.903610308.