PMID- 8551256
OWN - NLM
STAT- MEDLINE
DCOM- 19960220
LR  - 20190830
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 47
IP  - 1
DP  - 1995 Sep
TI  - Rapid genotyping of hepatitis C virus RNA-isolates obtained from patients 
      residing in western Europe.
PG  - 35-42
AB  - Two rapid genotyping methods for hepatitis C virus (HCV), the line probe assay 
      (Inno-LiPA) and the subtype-specific core amplification system [Okamoto et al., 
      (1992b) Journal of General Virology 73:673-679], were applied to 58 HCV isolates 
      which were typed as type 1 (n = 37) and type 2 (n = 21) by sequence analysis of 
      the 5' untranslated region (5'UTR). The line probe assay targets the 5'UTR and 
      recognized 12 subtype 1a, 25 subtype 1b, 18 subtype 2a, 2 subtype 2b and 1 
      subtype 2d in accordance with sequence analysis of this region. Subtype-specific 
      core amplification revealed 7 discrepancies among the 37 type 1 isolates when 
      compared to LiPA. A different subtype was observed in 3 isolates (1a versus 1b), 
      2 isolates remained untyped and 2 isolates showed a coinfection of subtype 1a and 
      1b. The first 5 discrepancies were confirmed by sequence analysis of the core 
      region whereas the coinfection could not be confirmed. Of the 21 type 2 isolates 
      only one could be typed by subtype-specific core amplification. HCV RNA was 
      detected in all 21 cases after the general first round of polymerase chain 
      reaction (PCR). Direct sequencing of the core region indicated sequence variation 
      as a source of failure. It is concluded that LiPA results are conclusive for 
      typing of HCV. However, LiPA is hampered occasionally for subtyping by lack of 
      subtype-specific sequence variation in 5'UTR. Subtyping results by 
      subtype-specific core amplification were accurate. However, it seems that this 
      assay is not suitable for the identification of genotype 2 isolates that 
      circulate in patients living in Western Europe.
FAU - Kleter, G E
AU  - Kleter GE
AD  - Department of Virology, Erasmus University Rotterdam, The Netherlands.
FAU - van Doorn, L J
AU  - van Doorn LJ
FAU - Stuyver, L
AU  - Stuyver L
FAU - Maertens, G
AU  - Maertens G
FAU - Brouwer, J T
AU  - Brouwer JT
FAU - Schalm, S W
AU  - Schalm SW
FAU - Heijtink, R A
AU  - Heijtink RA
FAU - Quint, W G
AU  - Quint WG
LA  - eng
SI  - GENBANK/X58937
SI  - GENBANK/X58938
SI  - GENBANK/X58939
SI  - GENBANK/X58940
SI  - GENBANK/X58941
SI  - GENBANK/X58942
SI  - GENBANK/X58943
SI  - GENBANK/X58944
SI  - GENBANK/X58945
SI  - GENBANK/X58946
SI  - GENBANK/X58947
SI  - GENBANK/X58948
SI  - GENBANK/X58949
SI  - GENBANK/X58950
SI  - GENBANK/X58951
SI  - GENBANK/X58952
SI  - GENBANK/X58953
SI  - GENBANK/X85560
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Belgium
MH  - DNA, Viral
MH  - Europe
MH  - Genotype
MH  - Hepacivirus/classification/*genetics/isolation & purification
MH  - Humans
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Netherlands
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/*blood
MH  - Sequence Homology, Nucleic Acid
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1002/jmv.1890470108 [doi]
PST - ppublish
SO  - J Med Virol. 1995 Sep;47(1):35-42. doi: 10.1002/jmv.1890470108.