PMID- 8551351
OWN - NLM
STAT- MEDLINE
DCOM- 19960222
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 16
IP  - 2
DP  - 1996 Jan 15
TI  - Brain-derived neurotrophic factor promotes the differentiation of various 
      hippocampal nonpyramidal neurons, including Cajal-Retzius cells, in organotypic 
      slice cultures.
PG  - 675-87
AB  - Brain-derived neurotrophic factor (BDNF) is widely expressed in the central 
      nervous system, where its function is poorly understood. The aim of this study 
      was to investigate the effects of BDNF on the differentiation of hippocampal 
      nonpyramidal neurons using organotypic slice cultures prepared from postnatal 
      rats. The application of BDNF induced an increase in immunostaining for the 
      microtubule-associated protein (MAP)-2 in non-pyramidal neurons of the stratum 
      oriens. BDNF promotes the elongation of the dendrites of these neurons, as 
      demonstrated by analysis after biocytin labeling. Calbindin-D- and 
      calretinin-containing subgroups of nonpyramidal cells in the stratum oriens were 
      responsive to BDNF but not to nerve growth factor, as shown by an increase in the 
      number of neurons immunostained for these proteins. BDNF also induced an increase 
      in neuropeptide Y immunostaining of stratum oriens neurons. In contrast, BDNF had 
      no effect on parvalbumin immunostaining, despite the fact that these cells 
      express the BDNF receptor trkB. In addition, BDNF increased calretinin 
      immunoreactivity in Cajal-Retzius cells situated around the hippocampal fissure. 
      The Cajal-Retzius neurons persisted in slices beyond the time at which they 
      degenerate in vivo. However, BDNF is not required for the survival of these 
      cells, because they also persisted in slices from BDNF knock-out mice. The 
      present results indicate that BDNF exerts an effect on the morphology of stratum 
      oriens nonpyramidal cells and their calcium-binding protein levels. BDNF also 
      regulates the calretinin content of Cajal-Retzius cells but is not necessary for 
      their survival.
FAU - Marty, S
AU  - Marty S
AD  - Department of Neurochemistry, Max Planck Institute for Psychiatry, Martinsried, 
      Germany.
FAU - Carroll, P
AU  - Carroll P
FAU - Cellerino, A
AU  - Cellerino A
FAU - Castren, E
AU  - Castren E
FAU - Staiger, V
AU  - Staiger V
FAU - Thoenen, H
AU  - Thoenen H
FAU - Lindholm, D
AU  - Lindholm D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured/drug effects
MH  - Hippocampus/*drug effects
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Proto-Oncogene Proteins c-fos/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6578634
EDAT- 1996/01/15 00:00
MHDA- 1996/01/15 00:01
PMCR- 1996/07/15
CRDT- 1996/01/15 00:00
PHST- 1996/01/15 00:00 [pubmed]
PHST- 1996/01/15 00:01 [medline]
PHST- 1996/01/15 00:00 [entrez]
PHST- 1996/07/15 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.16-02-00675.1996 [doi]
PST - ppublish
SO  - J Neurosci. 1996 Jan 15;16(2):675-87. doi: 10.1523/JNEUROSCI.16-02-00675.1996.