PMID- 8551709
OWN - NLM
STAT- MEDLINE
DCOM- 19960220
LR  - 20190817
IS  - 0021-5287 (Print)
IS  - 0021-5287 (Linking)
VI  - 86
IP  - 11
DP  - 1995 Nov
TI  - [Effects of castration on apoptosis and transforming growth factor-beta 1 mRNA in 
      the neonatal mouse seminal vesicles].
PG  - 1660-5
AB  - BACKGROUND: In the adult male rat prostate, castration induces apoptosis of 
      epithelial cells concomitant with the increase in transforming growth factor-beta 
      1 (TGF-beta 1). In the present study, we investigated the effects of castration 
      on apoptosis and TGF-beta 1 mRNA in neonatal mouse seminal vesicles. METHODS: 
      5-day-old BALB/c mice were castrated by Pfeifer's method. We estimated the 
      weight, 3H-thymidine uptake by whole seminal vesicles, the amount of TGF-beta 1 
      mRNA by RT-PCR method, and the apoptotic index of both epithelium and mesenchyme. 
      RESULTS: The castration of 5-day-old neonatal mice resulted in much less weight 
      of seminal vesicles and DNA synthesis estimated by 3H-thymidine uptake by whole 
      seminal vesicles compared to intact neonatal mice, indicating that the growth of 
      neonatal mouse seminal vesicles depends on androgens secreted by the testis. The 
      amount of TGF-beta 1 mRNA estimated by RT-PCR method increased 4 days after 
      castration at 5 days of age. However, the castration did not induce apoptosis in 
      the seminal vesicles. CONCLUSION: The present study indicates that castration of 
      neonatal mice does not induce apoptosis in the seminal vesicles, although it does 
      a transient increase in TGF-beta 1 mRNA in the seminal vesicles.
FAU - Tanji, N
AU  - Tanji N
AD  - Department of Urology, School of Medicine, Ehime University.
FAU - Rahman, S A
AU  - Rahman SA
FAU - Yokoyama, M
AU  - Yokoyama M
FAU - Takeuchi, M
AU  - Takeuchi M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Hinyokika Gakkai Zasshi
JT  - Nihon Hinyokika Gakkai zasshi. The japanese journal of urology
JID - 2984841R
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Apoptosis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Orchiectomy
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Seminal Vesicles/cytology/*metabolism
MH  - Transforming Growth Factor beta/*genetics
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.5980/jpnjurol1989.86.1660 [doi]
PST - ppublish
SO  - Nihon Hinyokika Gakkai Zasshi. 1995 Nov;86(11):1660-5. doi: 
      10.5980/jpnjurol1989.86.1660.