PMID- 8552404
OWN - NLM
STAT- MEDLINE
DCOM- 19960220
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 12
IP  - 1
DP  - 1996 Jan 4
TI  - Allelic deletion mapping on chromosome 5 in human carcinomas.
PG  - 97-102
AB  - We analysed allelic deletions on chromosome 5 in microdissected human non-small 
      cell lung cancers. Thirty-four primary squamous cell carcinomas, 15 primary 
      adenocarcinomas and five regional lymph node metastases were investigated for 
      loss of heterozygosity (LOH) in chromosomal region 5p15-q21. The sites analysed 
      included the APC tumor suppressor gene at 5q21, five polymorphic microsatellite 
      markers and the putative tumor suppressor locus del-27, that was assigned to 
      chromosomal region 5p13-12 by fluorescence in situ hybridization (FISH) analysis. 
      Allelic deletions encompassed larger genomic regions more often in squamous cell 
      carcinomas than in adenocarcinomas. The del-27 amd APC regions were identified as 
      two distinct regions with the highest LOH frequencies within 5p15-q21. In 
      squamous cell carcinomas LOH frequencies were 73% at the del-27 and 70% at the 
      APC locus. In adenocarcinomas LOH at the del-27 and APC loci occurred in 38% of 
      the informative cases. Allelic deletion of the APC gene and at the del-27 locus 
      was also detected in the metastases. The results suggest involvement of at least 
      two tumor suppressor genes on chromosome 5 in lung tumorigenesis.
FAU - Wieland, I
AU  - Wieland I
AD  - Institute for Cell Biology, (Cancer Research), University of Essen Medical 
      School, Germany.
FAU - Bohm, M
AU  - Bohm M
FAU - Arden, K C
AU  - Arden KC
FAU - Ammermuller, T
AU  - Ammermuller T
FAU - Bogatz, S
AU  - Bogatz S
FAU - Viars, C S
AU  - Viars CS
FAU - Rajewsky, M F
AU  - Rajewsky MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/secondary
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 5
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics
MH  - Molecular Sequence Data
EDAT- 1996/01/04 00:00
MHDA- 1996/01/04 00:01
CRDT- 1996/01/04 00:00
PHST- 1996/01/04 00:00 [pubmed]
PHST- 1996/01/04 00:01 [medline]
PHST- 1996/01/04 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1996 Jan 4;12(1):97-102.