PMID- 8554558
OWN - NLM
STAT- MEDLINE
DCOM- 19960222
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 217
IP  - 3
DP  - 1995 Dec 26
TI  - Induction of tyrosine phosphorylation and translocation of ezrin by hepatocyte 
      growth factor/scatter factor.
PG  - 1062-9
AB  - Ezrin is a member of the TERM family and is a key protein in cytoplasmic 
      membrane-cytoskeleton interactions. This study showed that hepatocyte growth 
      factor/scatter factor (HGF/SF), a cytokine known to regulate motility, 
      morphogenesis and growth of cells, stimulated the tyrosine phosphorylation of 
      ezrin in a human colon epithelial cell line, HT115. After HGF/SF stimulation, 
      ezrin translocated from the cytosol and generalised membrane to the areas of 
      ruffled membrane as visualised by indirect immunofluorescent and immunogold 
      electron microscopy. This effect was inhibited by genistein, a tyrosine kinase 
      inhibitor. It is concluded that HGF/SF induces ezrin translocation by stimulation 
      of its tyrosine phosphorylation and that this plays a key role in HGF/SF induced 
      membrane ruffling.
FAU - Jiang, W G
AU  - Jiang WG
AD  - University Department of Surgery, University of Wales College of Medicine, 
      Cardiff, UK.
FAU - Hiscox, S
AU  - Hiscox S
FAU - Singhrao, S K
AU  - Singhrao SK
FAU - Puntis, M C
AU  - Puntis MC
FAU - Nakamura, T
AU  - Nakamura T
FAU - Mansel, R E
AU  - Mansel RE
FAU - Hallett, M B
AU  - Hallett MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Actins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoflavones)
RN  - 0 (Phosphoproteins)
RN  - 0 (ezrin)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Cell Compartmentation
MH  - Cell Membrane/ultrastructure
MH  - Cytoskeletal Proteins
MH  - Enzyme Inhibitors/pharmacology
MH  - Genistein
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Isoflavones/pharmacology
MH  - Phosphoproteins/*metabolism
MH  - Phosphorylation
MH  - Phosphotyrosine/*metabolism
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors
MH  - Tumor Cells, Cultured
EDAT- 1995/12/26 00:00
MHDA- 1995/12/26 00:01
CRDT- 1995/12/26 00:00
PHST- 1995/12/26 00:00 [pubmed]
PHST- 1995/12/26 00:01 [medline]
PHST- 1995/12/26 00:00 [entrez]
AID - S0006291X8572877X [pii]
AID - 10.1006/bbrc.1995.2877 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Dec 26;217(3):1062-9. doi: 
      10.1006/bbrc.1995.2877.