PMID- 8555029
OWN - NLM
STAT- MEDLINE
DCOM- 19960227
LR  - 20190704
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 133
IP  - 5
DP  - 1995 Nov
TI  - Differentiation and CD23 expression of peripheral blood monocytes in patients 
      with atopic dermatitis.
PG  - 757-63
AB  - In this study we investigate the expression of the low affinity immunoglobulin E 
      (IgE) receptor (Fc epsilon RII) on plastic-adherent leucocytes from patients with 
      atopic dermatitis (AD). Peripheral blood mononuclear cells were obtained from 
      patients with AD, normal controls (and in one study from a group of atopic 
      asthmatic patients). Monocytes were separated by 2-h adherence to plastic. These 
      cells were then cultured for up to 7 days. Cells were harvested at time 0 (after 
      adherence), and after 5 and 7 days culture, and cytospins were prepared. The 
      proportion of cells expressing the phenotype of antigen presenting cells 
      (monoclonal antibodies (mAb) RFD1+), and mature phagocytes (mAb RFD7+) together 
      with CD23, were evaluated using combination staining with immunoperoxidase and 
      alkaline phosphatase anti-alkaline phosphatase methods. It was found that a 
      significantly larger proportion of circulating adherent cells in AD patients 
      expressed the RFD1 antigen, and that increases in the proportion of these 
      adherent cells expressing RFD1 or RFD7 occurred faster as cells matured in 
      culture, when compared with cells obtained from normal controls. By day 7, 
      however, equivalent proportions of RFD1+ and RFD7+ cells were present in AD and 
      control cultures. None the less, expression of the CD23 molecule was consistently 
      present on a larger proportion of both RFD1+ and RFD7+ cells from AD patients 
      compared with controls. The raised proportion of circulating RFD1+ cells found in 
      AD was not present in samples from atopic asthmatics, while the raised expression 
      of CD23 on these cells occurred in samples from both these groups. These data 
      support the suggestion that abnormalities in peripheral blood adherent cell 
      phenotype, maturation, and CD23 expression, occur in AD patients. Some of these 
      observations may be related to atopy in general rather than being specific for 
      this skin disease.
FAU - Buckley, C
AU  - Buckley C
AD  - Department of Dermatology, Royal Free Hospital School of Medicine, Hampstead, 
      London, U.K.
FAU - Rustin, M H
AU  - Rustin MH
FAU - Ivison, C
AU  - Ivison C
FAU - Poulter, L W
AU  - Poulter LW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Antigen-Presenting Cells/immunology
MH  - Asthma/immunology/pathology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dermatitis, Atopic/*immunology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Leukocytes, Mononuclear/*cytology/immunology
MH  - Phagocytes/immunology
MH  - Receptors, IgE/*immunology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1111/j.1365-2133.1995.tb02751.x [doi]
PST - ppublish
SO  - Br J Dermatol. 1995 Nov;133(5):757-63. doi: 10.1111/j.1365-2133.1995.tb02751.x.