PMID- 8556953
OWN - NLM
STAT- MEDLINE
DCOM- 19960227
LR  - 20071115
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 22
IP  - 3
DP  - 1995 Sep 15
TI  - Clinical, biological, and immunophenotypical characteristics of B-cell chronic 
      lymphocytic leukemia with trisomy 12 by fluorescence in situ hybridization.
PG  - 217-22
AB  - The clinical, biological, and immunophenotypical characteristics of B-cell 
      chronic lymphocytic leukemia (B-CLL) patients with trisomy 12 detected by 
      fluorescence in situ hybridization (FISH) using a chromosome 12 alpha-centromeric 
      probe (D12Z3) were analyzed in the present study. From a total of 104 consecutive 
      B-CLL patients, 21 (20%) displayed trisomy 12, the percentage of trisomic cells 
      ranging from 13% to 76%. From the clinico-biological point of view, patients with 
      trisomy 12 were associated with atypical CLL morphology (43% vs 10%, P = 0.04) 
      and BM diffuse pattern (75% vs. 25%, P = 0.02) together with increased WBC counts 
      (141 +/- 220 vs. 58 +/- 67 x 10(9)/L, P = 0.04). In contrast, no association was 
      detected between the presence of trisomy 12 and other disease characteristics 
      such as age, sex, clinical stage, hepatomegaly, lymphadenopathies, haemoglobin 
      levels and platelet counts, and the cell cycle distribution of PB leukocytes in 
      both groups of patients. Trisomy 12 patients had a significantly higher 
      expression of the FMC7 antigen both in percentage (34 +/- 34% vs. 13 +/- 20%, P = 
      0.02) and absolute numbers (29 +/- 62 vs. 7 +/- 17 x 10(9)/L, P = 0.007). No 
      major differences were found regarding the expression of mouse rosettes, CD19+, 
      and CD19+/CD5+ lymphocytes. Upon analyzing the correlations between the disease 
      characteristics of trisomy 12 cases, significant associations were found between 
      the percentage of trisomic cells and both the WBC count (r = 0.52, P = 0.02) and 
      the PB lymphocyte count (r = 0.60, P = 0.007).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Tabernero, M D
AU  - Tabernero MD
AD  - Unit of Cytometry, University of Salamanca, Spain.
FAU - San Miguel, J F
AU  - San Miguel JF
FAU - Garcia, J L
AU  - Garcia JL
FAU - Garcia-Isidoro, M
AU  - Garcia-Isidoro M
FAU - Wiegant, J
AU  - Wiegant J
FAU - Ciudad, J
AU  - Ciudad J
FAU - Gonzalez, M
AU  - Gonzalez M
FAU - Rios, A
AU  - Rios A
FAU - Raap, A
AU  - Raap A
FAU - Orfao, A
AU  - Orfao A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Cell Division
MH  - *Chromosomes, Human, Pair 12
MH  - DNA, Neoplasm
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/immunology/pathology
MH  - Male
MH  - Mice
MH  - *Trisomy
EDAT- 1995/09/15 00:00
MHDA- 1995/09/15 00:01
CRDT- 1995/09/15 00:00
PHST- 1995/09/15 00:00 [pubmed]
PHST- 1995/09/15 00:01 [medline]
PHST- 1995/09/15 00:00 [entrez]
AID - 10.1002/cyto.990220309 [doi]
PST - ppublish
SO  - Cytometry. 1995 Sep 15;22(3):217-22. doi: 10.1002/cyto.990220309.