PMID- 8557758
OWN - NLM
STAT- MEDLINE
DCOM- 19960226
LR  - 20181130
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 166
IP  - 1
DP  - 1996 Jan
TI  - Basic fibroblast growth factor stimulates hepatocyte growth factor/scatter factor 
      secretion by human mesenchymal cells.
PG  - 105-11
AB  - Basic fibroblast growth factor (bFGF) together with other pleiotropic factors 
      plays an important role in many complex physiological processes such as embryonic 
      development, angiogenesis, and wound repair. Among these factors, hepatocyte 
      growth factor/scatter factor (HGF/SF) which is secreted by cells of mesodermal 
      origin exerts its mito- and motogenic activities on cells of epithelial and 
      endothelial origin. Knowledge of the regulatory mechanisms of HGF/SF may 
      contribute to the understanding of its role in physio-pathological processes. We 
      observed that the secretion of HGF/SF by MRC-5 cells and by other 
      fibroblast-derived cell cultures in conditioned media was enhanced by exposure to 
      bFGF. HGF/SF was measured by the scatter assay, a bioassay for cell motility, and 
      was further characterized by Western blot analysis with anti-HGF/SF antibodies. 
      Exposure of MRC-5 cultures to 10 ng/ml of bFGF resulted already 6 h posttreatment 
      in a threefold higher amount of scatter factor secreted into the medium as 
      compared to untreated cultures. HGF/SF secretion was sustained after bFGF 
      treatment for the following 72 h when increased amounts of HGF/SF were detected 
      both in conditioned media as well as associated to the extracellular matrix. The 
      secretion of HGF/SF in cell supernatants increased dose dependently upon 
      treatment with bFGF starting from basal levels of 6 U/ml and reaching 27 U/ml at 
      30 ng/ml bFGF, plateauing thereafter. Upregulation of HGF/SF by IL-1, already 
      described by others, was confirmed in this study. Based on our findings an 
      articulated interaction can be speculated for bFGF, HGF/SF, and IL-1, e.g., in 
      tissue regeneration during inflammatory processes or in wound healing.
FAU - Roletto, F
AU  - Roletto F
AD  - Pharmacia Biopharmaceuticals-BioScience Center, Nerviano, Italy.
FAU - Galvani, A P
AU  - Galvani AP
FAU - Cristiani, C
AU  - Cristiani C
FAU - Valsasina, B
AU  - Valsasina B
FAU - Landonio, A
AU  - Landonio A
FAU - Bertolero, F
AU  - Bertolero F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Culture Media)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Cell Line/cytology
MH  - Collagenases/metabolism
MH  - Culture Media
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Hepatocyte Growth Factor/immunology/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 1
MH  - Mesoderm/*metabolism/physiology
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Signal Transduction/physiology
EDAT- 1996/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-4652(199601)166:1<105::AID-JCP12>3.0.CO;2-E [pii]
AID - 10.1002/(SICI)1097-4652(199601)166:1<105::AID-JCP12>3.0.CO;2-E [doi]
PST - ppublish
SO  - J Cell Physiol. 1996 Jan;166(1):105-11. doi: 
      10.1002/(SICI)1097-4652(199601)166:1<105::AID-JCP12>3.0.CO;2-E.