PMID- 8558713
OWN - NLM
STAT- MEDLINE
DCOM- 19960223
LR  - 20071114
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 155
IP  - 2
DP  - 1996 Feb
TI  - Evidence for a tumor suppressor gene distal to BRCA1 in prostate cancer.
PG  - 720-5
AB  - PURPOSE: The breast-ovarian cancer susceptibility gene, BRCA1, has been 
      implicated by both epidemiologic and genetic studies to be involved in prostate 
      cancer. We wished to test the frequency of BRCA1 deletion and that of other 
      markers in the region of proximal 17q in prostate tumor cells. MATERIALS AND 
      METHODS: We used a dual-color fluorescence in situ hybridization (FISH) assay 
      using P1 phage probes for the BRCA1 gene and 3 flanking sites at 17q12-21, as 
      well as a chromosome 17 centromere-specific alpha-satellite probe, to detect 
      deletions in single-cell suspensions and touch preparations from 23 primary 
      clinical stage B prostate tumors and adjacent nontumor prostate tissues. 
      Lymphoblastoid cells and prostate cells from a normal donor were used to 
      determine control loss values. RESULTS: Significant loss (p < 0.05) of at least 1 
      of the P1s was detected in 16 of 23 (70%) cases, and in 4 of those cases all 
      markers were lost, consistent with whole chromosome loss. Of the 12 cases with 
      subchromosomal loss, 8 had loss distal to BRCA1. Loss was detected in 5 cases 
      previously reported by using allelic imbalance (AI) methodologies, and was 
      detected in an additional 11 non-AI cases, suggesting that FISH is more sensitive 
      than AI for deletion detection in prostate tumor cells. CONCLUSIONS: The data 
      suggest that the region distal to BRCA1 may contain 1 or more prostate-specific 
      tumor suppressor genes and that BRCA1 itself plays only a minor role in prostate 
      cancer development.
FAU - Williams, B J
AU  - Williams BJ
AD  - Department of Pediatrics, University of Utah, Salt Lake City 84112, USA.
FAU - Jones, E
AU  - Jones E
FAU - Zhu, X L
AU  - Zhu XL
FAU - Steele, M R
AU  - Steele MR
FAU - Stephenson, R A
AU  - Stephenson RA
FAU - Rohr, L R
AU  - Rohr LR
FAU - Brothman, A R
AU  - Brothman AR
LA  - eng
GR  - 5P30 CA42014/CA/NCI NIH HHS/United States
GR  - R01-CA46269/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (BRCA1 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
CIN - J Urol. 1996 Feb;155(2):430-1. PMID: 8558628
MH  - BRCA1 Protein
MH  - Chromosome Mapping
MH  - *Gene Deletion
MH  - Genes, Tumor Suppressor/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Proteins/*genetics
MH  - Prostatic Neoplasms/*genetics
MH  - Transcription Factors/*genetics
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - S0022-5347(01)66509-1 [pii]
PST - ppublish
SO  - J Urol. 1996 Feb;155(2):720-5.