PMID- 8558918
OWN - NLM
STAT- MEDLINE
DCOM- 19960226
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 10
IP  - 1
DP  - 1996 Jan
TI  - Establishment of a human B-CLL xenograft model: utility as a preclinical 
      therapeutic model.
PG  - 130-7
AB  - Chronic lymphocytic leukemia (CLL), a proliferative disease of mature looking B 
      lymphocytes, is the commonest leukemia in western countries. It remains incurable 
      by available treatment modalities. We report on the establishment of a permanent, 
      EBV-negative, B-CLL line (WSU-CLL) from the peripheral blood of a patient with 
      CLL. The cells grow as suspension in liquid culture, express IgG lambda and other 
      B cell markers and show lg heavy and light gene rearrangements. Karyotypic 
      analysis shows 45,X,del(3)(p14;p24),t(4;12;12) (q31;q22;p13), t(5;12) (q31;p13), 
      add(16)(q24)X2, t(18;21) (q12;p12). WSU-CLL forms colonies when grown on soft 
      agar. A xenograft model was established by injecting the WSU-CLL cells 
      subcutaneously (s.c.) in severe combined immune deficient (SCID) mice. When the 
      s.c. tumor was transplanted in vivo to other SCID mice, the success rate was 100% 
      with a doubling time of 7.3 days. The CLL-SCID xenograft model was used to test 
      the efficacy of selected standard chemotherapy drugs and new therapeutic agents 
      against WSU-CLL. The cell line and the xenograft described can be used as a model 
      to facilitate the development of new therapeutic agents against CLL in man.
FAU - Mohammad, R M
AU  - Mohammad RM
AD  - Department of Internal Medicine, Wayne State University School of Medicine, 
      Detroit, MI 48201, USA.
FAU - Mohamed, A N
AU  - Mohamed AN
FAU - Hamdan, M Y
AU  - Hamdan MY
FAU - Vo, T
AU  - Vo T
FAU - Chen, B
AU  - Chen B
FAU - Katato, K
AU  - Katato K
FAU - Abubakr, Y A
AU  - Abubakr YA
FAU - Dugan, M C
AU  - Dugan MC
FAU - al-Katib, A
AU  - al-Katib A
LA  - eng
GR  - CA 22453/CA/NCI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Antineoplastic Agents)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Antineoplastic Agents/therapeutic use
MH  - Chromosome Aberrations
MH  - Disease Models, Animal
MH  - Drug Screening Assays, Antitumor
MH  - Humans
MH  - Immunophenotyping
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy/genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - Neoplasm Transplantation
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured/immunology/pathology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1996 Jan;10(1):130-7.