PMID- 8559754
OWN - NLM
STAT- MEDLINE
DCOM- 19960227
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 9
DP  - 1995 Sep
TI  - Chromosome 18 analysis by fluorescence in situ hybridization (FISH) in human 
      blastomeres of abnormal embryos after in vitro fertilization (IVF) attempt.
PG  - 835-41
AB  - We performed fluorescence in situ hybridization (FISH) with a chromosome 
      18-specific probe on human abnormal cleaved embryos, fertilized either by two 
      spermatozoa and exhibiting three pronuclei (3 PN) or normally fertilized and 
      exhibiting two pronuclei (2 PN) with subsequent severe fragmentation and/or 
      blocking. The aim of the study was to evaluate the incidence of chromosome 18 
      anomalies among these embryos in order to evaluate the FISH efficiency on such 
      material and to obtain more precise and complete data than those obtained with 
      classical cytogenetic analysis. For the 3 PN cleaved embryos, FISH confirmed the 
      frequent regulation towards diploidy (25 per cent) and the high frequency of 
      mosaics (53 per cent). For the 2 PN blocked or damaged embryos, FISH permitted 
      chromosome evaluation, which was otherwise impossible with classical cytogenetic 
      techniques: we also found a high mosaic frequency (45 per cent) with these 
      embryos. If this frequency were the same for normally developing embryos, it 
      would be a major obstacle to the reliability of either chromosomal or genetic 
      preimplantation diagnosis.
FAU - Bergere, M
AU  - Bergere M
AD  - IVF Laboratory, Hopital Antoine Beclere, Clamart, France.
FAU - Selva, J
AU  - Selva J
FAU - Baud, M
AU  - Baud M
FAU - Volante, M
AU  - Volante M
FAU - Martin, B
AU  - Martin B
FAU - Hugues, J N
AU  - Hugues JN
FAU - Olivennes, F
AU  - Olivennes F
FAU - Frydman, R
AU  - Frydman R
FAU - Auroux, M
AU  - Auroux M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Blastomeres
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 18
MH  - DNA Probes
MH  - Embryo, Mammalian/*abnormalities
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - In Vitro Techniques
MH  - Mosaicism/genetics
MH  - Ploidies
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1002/pd.1970150908 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Sep;15(9):835-41. doi: 10.1002/pd.1970150908.