PMID- 8561074
OWN - NLM
STAT- MEDLINE
DCOM- 19960223
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 105
IP  - 1
DP  - 1996 Jan
TI  - Genotypic analysis of primary head and neck squamous carcinoma by combined 
      fluorescence in situ hybridization and DNA flow cytometry.
PG  - 102-8
AB  - Concurrent DNA flow cytometric (FCM) and fluorescence in situ hybridization 
      (FISH) analyses were prospectively performed on 24 primary untreated head and 
      neck squamous carcinomas for characterization of the genotypic and phenotypic DNA 
      aberrations of these neoplasms. Eleven tumors (42.0%) manifested DNA diploidy (DI 
      = 1.00) and 15 (58.0%) had DNA aneuploidy (DI < or > 1.00) by FCM. Fluorescence 
      in situ hybridization results showed aneusomy in the majority of DNA diploid and 
      in all DNA aneuploid tumors. The extent of the abnormalities for individual 
      chromosomes and the number of involved chromosomes in a given DNA diploid or 
      aneuploid tumor were significantly different. Overall, a statistical correlation 
      between the FCM DNA index (DI) and the magnitude of the chromosomal aberration by 
      FISH was found. Our results also show a significant association between the DI 
      and histologic differentiation and stage of disease in these neoplasms. In 
      conclusion, (1) chromosomal aneusomy characterizes most DNA diploid (DI = 1.00) 
      and all DNA aneuploid (DI < or > 1.00) head and neck squamous carcinomas; (2) 
      polysomy is the most prevalent finding; (3) loss of the Y chromosome in tumors 
      from male patients is a consistent feature; (4) the FCM DI reflects net 
      chromosomal gains or losses in these neoplasms; and (5) DNA aneuploidy is 
      associated with tumor aggressiveness.
FAU - el-Naggar, A K
AU  - el-Naggar AK
AD  - Department of Pathology, University of Texas, M.D. Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Dinh, M
AU  - Dinh M
FAU - Tucker, S
AU  - Tucker S
FAU - Luna, M A
AU  - Luna MA
FAU - Goepfert, H
AU  - Goepfert H
FAU - Hsu, P
AU  - Hsu P
FAU - Batsakis, J G
AU  - Batsakis JG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - *Flow Cytometry
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Y Chromosome/pathology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1093/ajcp/105.1.102 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1996 Jan;105(1):102-8. doi: 10.1093/ajcp/105.1.102.