PMID- 8561969
OWN - NLM
STAT- MEDLINE
DCOM- 19960307
LR  - 20191101
IS  - 0893-7648 (Print)
IS  - 0893-7648 (Linking)
VI  - 11
IP  - 1-3
DP  - 1995 Aug-Dec
TI  - Prenatal cocaine exposure revealed minimal postnatal changes in rat striatal 
      dopamine D2 receptor sites and mRNA levels in the offspring.
PG  - 67-76
AB  - It has been reported from this laboratory that prenatal cocaine exposure results 
      in the postnatal transient alterations of rat striatal dopamine uptake sites 
      examined from postnatal 0-32 wk. The present study aims to examine whether this 
      will result in a direct/indirect stimulation of dopamine D2 receptors. Pregnant 
      rats were dosed orally with cocaine hydrochloride (60 mg/kg/d) from gestational 
      day (GD) 7-21. Control animals received an equivalent volume of water. The 
      striatum from the offspring at postnatal 0-32 wk was examined. The radioligand 
      [3H]sulpiride was used for the Scatchard analysis of the D2 receptors, and the 
      changes in the levels of mRNA for the D2 receptor were studied using Northern 
      blot analysis. Results from the present study revealed that in the control group, 
      there was an age-dependent increase in the number of D2 receptor sites (Bmax: 
      44.00 +/- 2.12 to 178.00 +/- 45.10 fmol/mg protein) and in the levels of D2 mRNA 
      from PN0-32 wk with the most rapid increase occurring during the first 4 wk of 
      postnatal development. Prenatal cocaine exposure resulting in only a significant 
      decrease (p < 0.001) in the number of D2 receptor sites at PN0 wk and in a 10% 
      increase in mRNA levels at PN3, 4, and 12 wk. It was concluded from this study 
      that prenatal cocaine exposure resulted in minimal postnatal changes in the 
      dopamine D2 receptor.
FAU - Stadlin, A
AU  - Stadlin A
AD  - Department of Anatomy, Chinese University of Hong Kong, Shatin, New Territories, 
      Hong Kong.
FAU - Choi, H L
AU  - Choi HL
FAU - Tsim, K W
AU  - Tsim KW
FAU - Tsang, D
AU  - Tsang D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Dopamine D2)
RN  - 7MNE9M8287 (Sulpiride)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Blotting, Northern
MH  - Cocaine/*pharmacology
MH  - Corpus Striatum/drug effects/growth & development/*metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Gestational Age
MH  - Kinetics
MH  - Litter Size/drug effects
MH  - Male
MH  - Pregnancy
MH  - Pregnancy, Animal/*drug effects
MH  - *Prenatal Exposure Delayed Effects
MH  - RNA, Messenger/*metabolism
MH  - Radioligand Assay
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Dopamine D2/biosynthesis/drug effects/*metabolism
MH  - Reference Values
MH  - Sulpiride/metabolism
MH  - Weight Gain/drug effects
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1007/BF02740685 [doi]
PST - ppublish
SO  - Mol Neurobiol. 1995 Aug-Dec;11(1-3):67-76. doi: 10.1007/BF02740685.