PMID- 8564092
OWN - NLM
STAT- MEDLINE
DCOM- 19960306
LR  - 20220330
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 153
IP  - 2
DP  - 1996 Feb
TI  - Differences in interleukin-8 and tumor necrosis factor-alpha in induced sputum 
      from patients with chronic obstructive pulmonary disease or asthma.
PG  - 530-4
AB  - Asthma and chronic obstructive pulmonary disease are characterized by chronic 
      airway inflammation. Studies using bronchoalveolar lavage (BAL) have shown an 
      increased proportion of eosinophils in the BAL fluid from asthmatics compared 
      with that from normal subjects, whereas studies of chronic obstructive pulmonary 
      disease (COPD) have shown increased numbers of neutrophils. Induced sputum allows 
      sampling of respiratory tract secretions from patients and control subjects, 
      providing a noninvasive method of studying airway secretions and allowing 
      characterization of cells and measurement of soluble markers. We investigated 
      whether induced sputum was a useful method of studying airway fluid from patients 
      with moderate to severe COPD and whether it could be used to compare inflammation 
      in this condition with that in asthma. An initial reproducibility study was 
      undertaken. Sputum was induced twice in 13 patients with severe COPD at a 14-d 
      interval. Total and differential cell counts were carried out and were found to 
      be reproducible over this period. Sputum was then induced in 14 patients with 
      COPD, 23 patients with asthma, 12 healthy cigarette smokers, and 16 normal 
      nonsmoking control subjects. We found a significant increase in neutrophils and 
      increased concentrations of tumor necrosis factor-alpha (TNF alpha) and 
      interleukin-8 (IL-8) in the patients with COPD compared with the smoking and 
      nonsmoking control subjects. Interleukin-8, but not TNF alpha, was significantly 
      higher in the COPD group than in the asthmatic group. We conclude that the 
      cytokines TNF alpha and IL-8 may be involved in the inflammation in COPD.
FAU - Keatings, V M
AU  - Keatings VM
AD  - Department of Thoracic Medicine, National Heart and Lung Institute, London, 
      United Kingdom.
FAU - Collins, P D
AU  - Collins PD
FAU - Scott, D M
AU  - Scott DM
FAU - Barnes, P J
AU  - Barnes PJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Asthma/*metabolism/pathology
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Eosinophils
MH  - Female
MH  - Humans
MH  - Inflammation
MH  - Interleukin-8/*analysis
MH  - Leukocyte Count
MH  - Lung Diseases, Obstructive/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neutrophils
MH  - Reproducibility of Results
MH  - Smoking/metabolism/pathology
MH  - Sputum/*chemistry/cytology
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1164/ajrccm.153.2.8564092 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1996 Feb;153(2):530-4. doi: 
      10.1164/ajrccm.153.2.8564092.