PMID- 8565269
OWN - NLM
STAT- MEDLINE
DCOM- 19960301
LR  - 20190512
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 103
IP  - 1
DP  - 1996 Jan
TI  - Differential spontaneous expression of mRNA for IL-4, IL-10, IL-13, IL-2 and 
      interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMC) from 
      atopic patients.
PG  - 111-8
AB  - Distinct cytokine-producing T cell subsets are well known to play a major role in 
      IgE production and to be differentially regulated in allergic patients, although 
      the characterization of the type 1/type 2 cytokine pattern in PBMC during 
      allergic responses remains to be clearly defined. The aim of this study was to 
      determine whether different cytokine profiles are observed directly in PBMC of 
      atopic donors. We attempted to study several cytokines (IL-2, IFN-gamma, IL-4, 
      IL-10 and IL-13) using not only ELISA but also polymerase chain reaction (PCR) 
      techniques, because the frequency of cytokine-producing cells in peripheral blood 
      is very low. All the patients were selected during their acute symptomatologic 
      phase. Data showed a significantly higher production of IL-4 (P = 0.05) and IL-10 
      (P < 0.005) as determined by ELISA in phytohaemagglutinin (PHA)/phorbol myristate 
      acetate (PMA)-stimulated mononuclear cells of atopic donors compared with 
      controls, although spontaneous IL-4 production without stimulation was never 
      detected within either atopic or control groups. The reverse-transcriptase 
      (RT)-PCR technique appeared to be advantageous in that it allowed the detection 
      of the spontaneous expression of cytokine mRNA in cells without stimulation. We 
      found a clear expression of IL-4 mRNA spontaneously in all atopic patients, 
      whereas normal donors in most cases did not show specific signals (P < 0.0001). 
      Less differences between atopic subjects and controls were found in IL-10 mRNA 
      expression. Although the technique of RT-PCR amplification used in this study is 
      semiquantitative, a reproducible and significant (P < 0.001) enhancement of IL-10 
      mRNA expression was observed in atopic donors. A heterogeneous expression of 
      IL-13 mRNA was observed in individuals from the two groups studied, although mean 
      levels in atopic donors were slightly enhanced compared with controls (P = 0.02). 
      Furthermore, we did not observe any alteration in the expression of the type 
      1-derived cytokines such as IFN-gamma and IL-2. In addition, we showed a lack of 
      correlation between the expression of serum IgE (total or specific) and 
      spontaneous IL-4 mRNA expression. This study showed a tendency of PBMC from 
      atopic donors to express a type 2-like cytokine pattern, with IL-4 as the most 
      discriminatory cytokine. Additionally, as the level of serum IgE has a low 
      predictive value in allergic disease, and as the elevated expression of IL-4 that 
      we found was not correlated with serum IgE, we could strongly suggest that the 
      measurement of IL-4 in blood mononuclear cells may be of great value in the 
      analysis of allergic responses in atopic donors.
FAU - Esnault, S
AU  - Esnault S
AD  - Laboratoire d'Immunologie et d'Hematologie, Universite de Reims, France.
FAU - Benbernou, N
AU  - Benbernou N
FAU - Lavaud, F
AU  - Lavaud F
FAU - Shin, H C
AU  - Shin HC
FAU - Potron, G
AU  - Potron G
FAU - Guenounou, M
AU  - Guenounou M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Interleukins)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Immediate/blood/*immunology
MH  - Immunoglobulin E/blood
MH  - Interferon-gamma/*biosynthesis/blood/genetics
MH  - Interleukins/*biosynthesis/blood/genetics
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - RNA, Messenger/*biosynthesis
PMC - PMC2200319
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
PMCR- 1997/01/01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PHST- 1997/01/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1996.00911.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1996 Jan;103(1):111-8. doi: 10.1046/j.1365-2249.1996.00911.x.