PMID- 8565700
OWN - NLM
STAT- MEDLINE
DCOM- 19960306
LR  - 20190830
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 104
IP  - 4
DP  - 1995 Dec
TI  - Molecular characterization and cytogenetic analysis of highly repeated DNAs of 
      lake trout, Salvelinus namaycush.
PG  - 242-51
AB  - The chromosomes of lake trout (Salvelinus namaycush) contain a considerable 
      amount of heterochromatin located at the centromeres and/or telomeres of several 
      chromosomes, including a sex-specific block located distally on the X chromosome. 
      In order to investigate further the repetitive DNAs of lake trout, genomic DNA 
      from a female was size fractionated (<600 bp) with the restriction endonuclease 
      AluI and fragments were cloned into the bacteriophage M13. A total of 42 clones 
      were isolated. Relative copy number of individual inserts within the lake trout 
      genome was estimated by Southern analysis. Twelve clones were determined to be 
      highly repetitive and were chosen for further investigation. Inserts of these 
      clones contained sequences similar to the AluI/RsaI, EcoRI/DraI, DraI/BstEII, and 
      MboI/BglII families reported from Arctic char (Salvelinus alpinus). The 
      chromosomal location of several of these fragments was determined in lake trout 
      by fluorescence in situ hybridization (FISH). Two related AluI/RsaI sequences 
      (Type A, approximately 140 bp, and Type B,approximately 120 bp) showed 
      differential hybridization. Type A hybridized to the centromeres of all 
      metacentric as well as several acrocentric chromosomes. Type B hybridized to the 
      centromeres of most acrocentric chromosomes. A sequence with homology to the 
      EcoRI/DraI family hybridized to the centromeres of several acrocentric 
      chromosomes. Sequences with partial similarity to the DraI/BstEII family 
      hybridized to the major rDNA sites (nucleolar organizer regions, NORs) and 
      several minor telomeric sites. The interstitial and telomeric heterochromatin of 
      lake trout, including that of the X chromosome, appears to comprise sequences 
      belonging to the MboI/BglII family.
FAU - Reed, K M
AU  - Reed KM
AD  - Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, 
      WI 53201, USA.
FAU - Phillips, R B
AU  - Phillips RB
LA  - eng
SI  - GENBANK/U27086
SI  - GENBANK/U27087
SI  - GENBANK/U27088
SI  - GENBANK/U27089
SI  - GENBANK/U27090
SI  - GENBANK/U27091
SI  - GENBANK/U27092
SI  - GENBANK/U27093
SI  - GENBANK/U27094
SI  - GENBANK/U27095
SI  - GENBANK/U27096
SI  - GENBANK/U27097
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - *Cytogenetics
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Probe Techniques
MH  - Molecular Sequence Data
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Species Specificity
MH  - Trout/*genetics
MH  - X Chromosome/*genetics
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1007/BF00352255 [doi]
PST - ppublish
SO  - Chromosoma. 1995 Dec;104(4):242-51. doi: 10.1007/BF00352255.