PMID- 8566835
OWN - NLM
STAT- MEDLINE
DCOM- 19960304
LR  - 20190501
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 38
IP  - 1
DP  - 1996 Jan
TI  - Effect of c-kit ligand, stem cell factor, on mediator release by human intestinal 
      mast cells isolated from patients with inflammatory bowel disease and controls.
PG  - 104-14
AB  - The regulation of mediator release in human intestinal mast cells is largely 
      unknown. Apart from IgE receptor crosslinking no secretagogues have been 
      described so far. This study examined the effect of two cytokines (c-kit ligand 
      and interleukin 3) and other agonists on human intestinal mast cell function. 
      Cells were isolated from surgery specimens of 47 patients undergoing intestinal 
      resection because of tumours or inflammatory bowel disease. Cell suspensions 
      contained 3.6% mast cells (mean of 50 experiments). After preincubation without 
      or with c-kit ligand or interleukin 3, cells were stimulated by IgE receptor 
      crosslinking, C5a or formyl-methionyl-leucyl-phenylalanine (fMLP). Histamine and 
      sulphidoleukotriene release was measured in supernatants. The sequential 
      stimulation of the cells with c-kit ligand and IgE receptor crosslinking induced 
      the release of high amounts of histamine and leukotrienes, whereas each agonist 
      by itself induced only marginal mediator release. Interleukin 3 induced no 
      release by itself, but enhanced the IgE receptor dependent release, possibly by 
      an indirect mechanism. No significant mediator release was seen in response to 
      C5a and fMLP, even if the cells were pretreated with c-kit ligand. The mediator 
      release, particularly that of leukotrienes, was higher in cells isolated from 
      actively inflamed tissue from patients with inflammatory bowel disease compared 
      with controls. In conclusion, it was found that, apart from IgE receptor 
      crosslinking, c-kit ligand and interleukin 3 regulate mediator release in human 
      intestinal mast cells. The enhancement of mediator release by cytokines may be of 
      particular relevance in the pathogenesis of inflammatory bowel diseases and food 
      intolerance reactions.
FAU - Bischoff, S C
AU  - Bischoff SC
AD  - Department of Gastroenterology and Hepatology, Medical School of Hannover, 
      Germany.
FAU - Schwengberg, S
AU  - Schwengberg S
FAU - Wordelmann, K
AU  - Wordelmann K
FAU - Weimann, A
AU  - Weimann A
FAU - Raab, R
AU  - Raab R
FAU - Manns, M P
AU  - Manns MP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Interleukin-3)
RN  - 0 (Leukotrienes)
RN  - 0 (Ligands)
RN  - 0 (Stem Cell Factor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Female
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - Inflammatory Bowel Diseases/*metabolism
MH  - Interleukin-3/*pharmacology
MH  - Leukotrienes/metabolism
MH  - Ligands
MH  - Male
MH  - Mast Cells/*metabolism
MH  - Middle Aged
MH  - Stem Cell Factor/*pharmacology
PMC - PMC1382987
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
PMCR- 1996/01/01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PHST- 1996/01/01 00:00 [pmc-release]
AID - 10.1136/gut.38.1.104 [doi]
PST - ppublish
SO  - Gut. 1996 Jan;38(1):104-14. doi: 10.1136/gut.38.1.104.