PMID- 8567769
OWN - NLM
STAT- MEDLINE
DCOM- 19960301
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 10
IP  - 10
DP  - 1995 Oct
TI  - Time-dependent effects of transforming growth factor alpha on aromatase activity 
      in human granulosa cells.
PG  - 2554-9
AB  - Transforming growth factor alpha (TGF alpha) is implicated as a paracrine growth 
      factor in the regulation of human granulosa cell function. To investigate this 
      further, we have examined the actions of TGF alpha on the basal and 
      follicle-stimulating hormone (FSH)-stimulated aromatase activity of human 
      granulosa cells to determine how this growth factor influences oestrogen 
      biosynthesis in the follicle. Granulosa cells from women having in-vitro 
      fertilization during untreated or gonadotrophin-stimulated cycles were cultured 
      for 1-6 days in the presence or absence of FSH or TGF alpha at a range of doses. 
      Aromatase activity, expressed as oestradiol production, was determined after 
      culture during a 3 h test period. After 2 days, TGF alpha (1-300 ng/ml) decreased 
      basal and FSH-stimulated aromatase activity in a dose-dependent manner (ED50 = 3 
      ng/ml). In contrast, after 4 days, TGF alpha enhanced both basal and 
      FSH-stimulated aromatase activity. Repeated experiments revealed a consistent 
      pattern of inhibition on day 2, which was more marked in the presence of FSH 
      (reduction by 30.6 +/- 9.1%, mean +/- SEM; n = 14; P < 0.01), and stimulation on 
      day 4 in both the absence (increased by 61.4 +/- 20.6%, mean +/- SEM; n = 6; P < 
      0.05) and presence of FSH (increased by 36.0 +/- 15.2%, mean +/- SEM; n = 8; P < 
      0.05). The results provide further evidence that TGF alpha is a paracrine factor 
      in the control of oestrogen biosynthesis, but the actions can be either 
      inhibitory or stimulatory depending on the duration of exposure.
FAU - Harlow, C R
AU  - Harlow CR
AD  - University of Bristol, Department of Obstetrics and Gynaecology, St Michael's 
      Hospital, UK.
FAU - Cahill, D J
AU  - Cahill DJ
FAU - Maile, L A
AU  - Maile LA
FAU - Wardle, P G
AU  - Wardle PG
FAU - Hull, M G
AU  - Hull MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Transforming Growth Factor alpha)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Aromatase/*metabolism
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/biosynthesis
MH  - Female
MH  - Fertilization in Vitro
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Granulosa Cells/drug effects/*enzymology
MH  - Humans
MH  - Kinetics
MH  - Transforming Growth Factor alpha/administration & dosage/*pharmacology
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a135744 [doi]
PST - ppublish
SO  - Hum Reprod. 1995 Oct;10(10):2554-9. doi: 10.1093/oxfordjournals.humrep.a135744.