PMID- 8568765
OWN - NLM
STAT- MEDLINE
DCOM- 19960306
LR  - 20190724
IS  - 0022-4251 (Print)
IS  - 0022-4251 (Linking)
VI  - 105
IP  - 2
DP  - 1995 Nov
TI  - Maintenance of bovine oocytes in prophase of meiosis I by high [cAMP]i.
PG  - 227-35
AB  - The effects of high intracellular cAMP concentrations ([cAMP]i) on germinal 
      vesicle maintenance of bovine cumulus-oocyte complexes were investigated, using 
      8-bromo-3',5'-cAMP (8-Br-cAMP) or an invasive adenylate cyclase from Bordetella 
      pertussis to increase the [cAMP]i. The effects of interactions of these agents 
      with macromolecular supplements in culture medium (fetal calf serum, FCS; 
      polyvinylpyrrolidone, PVP; BSA), and different methods of processing complexes 
      before culture, on subsequent germinal vesicle maintenance by invasive adenylate 
      cyclase were studied. While 8-Br-cAMP was unable to maintain germinal vesicle 
      arrest in the majority of oocytes for 20 h (36% with FCS, 24% with BSA, 18% with 
      PVP), it maintained germinal vesicle arrest in a high proportion of 
      cumulus-enclosed oocytes when BSA or PVP was used (37% with FCS, 52% with BSA, 
      53% with PVP). The difference in frequency of germinal vesicle maintenance 
      between macromolecular supplements was not related to [cAMP]i when assayed after 
      culture for 2 h with invasive adenylate cyclase. Complexes processed in whole 
      follicular fluid were not maintained in meiotic arrest (26%) when cultured with 
      invasive adenylate cyclase and PVP. Complexes processed in follicular fluid with 
      3-isobutyl 1-methylxanthine (IBMX) plus invasive adenylate cyclase were arrested 
      at the germinal vesicle stage at high frequencies (65%), while those processed in 
      IBMX or IBMX plus 8-Br-cAMP-supplemented follicular fluid had intermediate (43% 
      and 49%, respectively) frequencies of intact germinal vesicles. Oocyte complexes 
      processed in follicular fluid supplemented with IBMX and invasive adenylate 
      cyclase formed morulae and blastocysts (27.2%), as did oocytes processed in 
      follicular fluid alone (26%). Phosphoprotein profiles showed that control oocytes 
      and 8-Br-cAMP-treated oocytes share a profile that is different from that of 
      oocytes treated with invasive adenylate cyclase. These results show that 
      increased [cAMP]i reversibly maintains bovine oocytes in meiotic arrest for an 
      extended period without the occurrence of the post-translational protein 
      modifications observed during meiotic resumption or transient arrest.
FAU - Aktas, H
AU  - Aktas H
AD  - Department of Meat and Animal Science, University of Wisconsin, Madison 53706, 
      USA.
FAU - Wheeler, M B
AU  - Wheeler MB
FAU - Rosenkrans, C F Jr
AU  - Rosenkrans CF Jr
FAU - First, N L
AU  - First NL
FAU - Leibfried-Rutledge, M L
AU  - Leibfried-Rutledge ML
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Reprod Fertil
JT  - Journal of reproduction and fertility
JID - 0376367
RN  - 0 (Culture Media)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Adenylyl Cyclases/pharmacology
MH  - Animals
MH  - Bordetella pertussis
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Culture Media
MH  - Cyclic AMP/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Oocytes/*cytology
MH  - *Prophase
MH  - Serum Albumin, Bovine/pharmacology
MH  - Specimen Handling
EDAT- 1995/11/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1530/jrf.0.1050227 [doi]
PST - ppublish
SO  - J Reprod Fertil. 1995 Nov;105(2):227-35. doi: 10.1530/jrf.0.1050227.