PMID- 8569761
OWN - NLM
STAT- MEDLINE
DCOM- 19960306
LR  - 20191210
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 151
IP  - 2
DP  - 1995 Oct 18
TI  - Genomic cloning and chromosomal assignment of rat regucalcin gene.
PG  - 157-63
AB  - The gene for a Ca(2+)-binding protein regucalcin was cloned from a rat genomic 
      library which was constructed in lambda FIX II by screening with radiolabeled 
      probe (complementary DNA of rat liver regucalcin). Positive clone had 19.9 kb 
      insert of size and contained four exons of the gene coding for a rat regucalcin. 
      These exons included the partial coding sequence (61.2% of open reading frame) 
      and the entire 3'-untranslated region of the gene. The nucleotide sequence of 
      exons completely agreed with that of a rat regucalcin cDNA clone. The sequence 
      analysis of the clone showed that the identifier sequence and two simple repeated 
      sequences exist in the intron of the gene. Moreover, chromosomal location of the 
      rat regucalcin gene was determined by direct R-banding fluorescence in situ 
      hybridization (FISH) method with the 19.9 kb clone containing four exons. The 
      regucalcin gene was localized on rat chromosome Xq11.1-12 proximal end.
FAU - Shimokawa, N
AU  - Shimokawa N
AD  - Laboratory of Endocrinology and Molecular Metabolism, Graduate School of 
      Nutritional Sciences, University of Shizuoka, Japan.
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Yamaguchi, M
AU  - Yamaguchi M
LA  - eng
SI  - GENBANK/D31662
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA Probes)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RGN protein, human)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.2 (alcohol sulfotransferase)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.- (Rgn protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Binding Proteins/chemistry/*genetics
MH  - Carboxylic Ester Hydrolases
MH  - Chromosome Banding
MH  - *Cloning, Molecular
MH  - DNA Probes
MH  - Exons/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Intracellular Signaling Peptides and Proteins
MH  - Molecular Sequence Data
MH  - Rats
MH  - Restriction Mapping
MH  - Sex Chromosomes/genetics
MH  - Sulfotransferases
MH  - X Chromosome/*genetics
EDAT- 1995/10/18 00:00
MHDA- 1995/10/18 00:01
CRDT- 1995/10/18 00:00
PHST- 1995/10/18 00:00 [pubmed]
PHST- 1995/10/18 00:01 [medline]
PHST- 1995/10/18 00:00 [entrez]
AID - 10.1007/BF01322338 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1995 Oct 18;151(2):157-63. doi: 10.1007/BF01322338.