PMID- 8569785
OWN - NLM
STAT- MEDLINE
DCOM- 19960305
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 349
IP  - 1
DP  - 1996 Jan 17
TI  - Detection of chromosomal aberrations by fluorescence in situ hybridization in the 
      first three postirradiation divisions of human lymphocytes.
PG  - 127-35
AB  - Chromosomal aberrations in human lymphocytes were analyzed by fluorescence in 
      situ hybridization (FISH) in the first 3 postirradiation (0 and 2 Gy) divisions. 
      Cells were grown in the presence of BrdU, collected at different sampling times 
      (47, 70 and 91 h) and analyzed using an alphoid centromeric probe and PCR 
      amplified DNA libraries for chromosomes 2 and 8. Following differential staining 
      of sister chromatids, the analyzed cells were identified to be either in the 
      first, second or third mitosis after irradiation. The frequencies of both 
      dicentrics and fragments showed a reduction of about 50% after each cell 
      generation, whereas translocations were more persistent. Cells within the same 
      postirradiation division showed higher aberration frequencies when derived from 
      later sampling times, indicating a delay in progression of aberrant cells. As a 
      result, the frequencies for dicentrics and fragments remained rather constant at 
      different sampling times if the cell cycle parameter was not taken into account. 
      Thus, the average generation time of the lymphocytes had a clear effect on the 
      obtained aberration frequencies. The described method allows the study of the 
      persistence of chromosome damage using the FISH technique during 3 subsequent 
      cell divisions in vitro.
FAU - Boei, J J
AU  - Boei JJ
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      Netherlands.
FAU - Vermeulen, S
AU  - Vermeulen S
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Probes)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/cytology/*radiation effects
MH  - Male
MH  - Mitosis
MH  - Molecular Sequence Data
EDAT- 1996/01/17 00:00
MHDA- 1996/01/17 00:01
CRDT- 1996/01/17 00:00
PHST- 1996/01/17 00:00 [pubmed]
PHST- 1996/01/17 00:01 [medline]
PHST- 1996/01/17 00:00 [entrez]
AID - 0027510795001719 [pii]
AID - 10.1016/0027-5107(95)00171-9 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Jan 17;349(1):127-35. doi: 10.1016/0027-5107(95)00171-9.