PMID- 8569802
OWN - NLM
STAT- MEDLINE
DCOM- 19960301
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 359
IP  - 1
DP  - 1996 Jan 16
TI  - Cytogenetic analysis of mice chronically fed the food mutagen 
      2-amino-1-methyl-6-phenylimidazo[4,5b]pyridine.
PG  - 53-61
AB  - The cytogenetic effects in mice chronically fed the heterocyclic amine 
      2-amino-1-methyl-6-phenylimidazo[4,5b]pyridine (PhIP) were evaluated by 
      chromosome painting, micronucleated normochromatic erythrocytes (MN NCEs) and 
      sister chromatid exchanges (SCEs). PhIP and numerous other heterocyclic amines 
      have been isolated from cooked foods, and many have been found to be carcinogenic 
      in laboratory rodents. Female C57BL/6N mice were chronically fed a diet 
      containing 0, 100, 250 or 400 ppm of PhIP beginning at 8 weeks of age. Peripheral 
      blood and bone marrow were taken from 5 mice per treatment group at 1, 4 and 6 
      months from the start of exposure. PhIP was removed from the diet for a final 
      month of the experiment, at which time blood was taken from the remaining 
      animals. Chromosome-specific composite DNA probes for mouse chromosomes 2 and 8 
      were hybridized to metaphase cells from each tissue. The 1- and 4-month time 
      points showed no statistically significant difference between the control and 
      exposed mice for either tissue in chromosome aberration frequencies. Both MN NCEs 
      and SCEs were analyzed at a single time point during exposure (4 months for MN 
      NCEs and 6 months for SCEs) and again 1 month after removing PhIP from the diet. 
      MN NCEs in the peripheral blood showed a statistically significant dose response, 
      with all values decreasing significantly 1 month after removing PhIP from the 
      diet. SCE frequencies in the peripheral blood showed an approximate doubling 
      compared to control mice, and decreased to control levels 1 month after removing 
      PhIP from the diet. SCE frequencies in the bone marrow of exposed mice showed no 
      difference from the control animals. These results show that chronic ingestion of 
      PhIP by female C57BL/6 mice does not produce persistent cytogenetic damage as 
      visualized by chromosome aberrations, MN NCEs or SCEs.
FAU - Director, A E
AU  - Director AE
AD  - Genetics and Developmental Biology Program, College of Agriculture and Forestry, 
      West Virginia University, Morgantown 26506-6108, USA.
FAU - Nath, J
AU  - Nath J
FAU - Ramsey, M J
AU  - Ramsey MJ
FAU - Swiger, R R
AU  - Swiger RR
FAU - Tucker, J D
AU  - Tucker JD
LA  - eng
GR  - CA55861/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Imidazoles)
RN  - 0 (Mutagens)
RN  - 909C6UN66T (2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Diet
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Food/toxicity
MH  - Imidazoles/*toxicity
MH  - Lymphocytes/*drug effects/ultrastructure
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Micronucleus Tests
MH  - Mutagens/*toxicity
MH  - *Sister Chromatid Exchange
EDAT- 1996/01/16 00:00
MHDA- 1996/01/16 00:01
CRDT- 1996/01/16 00:00
PHST- 1996/01/16 00:00 [pubmed]
PHST- 1996/01/16 00:01 [medline]
PHST- 1996/01/16 00:00 [entrez]
AID - S0165-1161(96)90009-6 [pii]
AID - 10.1016/s0165-1161(96)90009-6 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Jan 16;359(1):53-61. doi: 10.1016/s0165-1161(96)90009-6.