PMID- 8571554 OWN - NLM STAT- MEDLINE DCOM- 19960305 LR - 20190830 IS - 0165-2427 (Print) IS - 0165-2427 (Linking) VI - 47 IP - 3-4 DP - 1995 Aug TI - Partial sequence of the equine immunoglobulin epsilon heavy chain cDNA. PG - 363-7 AB - In order to isolate a part of the immunoglobulin E (IgE) heavy chain cDNA of the horse, primers have been designed based upon well conserved sequences in humans, sheep and rats. The PCR resulted in a 500 bp fragment which hybridised with a human IgE constant region probe. The fragment was cloned and sequenced and its derived protein sequence compared with the corresponding sequences in humans, sheep and mice. Most amino acids common to these three species are also shared by the horse. FAU - Marti, E AU - Marti E AD - Klinik fur Nutztiere und Pferde, Berne, Switzerland. FAU - Szalai, G AU - Szalai G FAU - Bucher, K AU - Bucher K FAU - Dobbelaere, D AU - Dobbelaere D FAU - Gerber, H AU - Gerber H FAU - Lazary, S AU - Lazary S LA - eng SI - GENBANK/L31502 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Vet Immunol Immunopathol JT - Veterinary immunology and immunopathology JID - 8002006 RN - 0 (DNA Primers) RN - 0 (DNA, Complementary) RN - 0 (Immunoglobulin epsilon-Chains) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cloning, Molecular MH - Conserved Sequence MH - DNA Primers/chemistry MH - DNA, Complementary/*analysis/chemistry MH - Horses/*genetics/immunology MH - Humans MH - Immunoglobulin E/chemistry/*genetics MH - Immunoglobulin epsilon-Chains/chemistry/*genetics MH - Mice MH - Molecular Sequence Data MH - Polymerase Chain Reaction/veterinary MH - Rats MH - Sequence Homology, Amino Acid MH - Sheep EDAT- 1995/08/01 00:00 MHDA- 1995/08/01 00:01 CRDT- 1995/08/01 00:00 PHST- 1995/08/01 00:00 [pubmed] PHST- 1995/08/01 00:01 [medline] PHST- 1995/08/01 00:00 [entrez] AID - 0165-2427(94)05404-G [pii] AID - 10.1016/0165-2427(94)05404-g [doi] PST - ppublish SO - Vet Immunol Immunopathol. 1995 Aug;47(3-4):363-7. doi: 10.1016/0165-2427(94)05404-g.