PMID- 8572593
OWN - NLM
STAT- MEDLINE
DCOM- 19960301
LR  - 20131121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 15
IP  - 5B
DP  - 1995 Sep-Oct
TI  - Maintenance of human hyperplastic prostate implants at different sites in athymic 
      mice.
PG  - 2007-11
AB  - The present study determined the influence of implantation sites, androgens, and 
      the graft's fibrovascular elements on the maintenance of epithelial elements of 
      human benign hyperplastic prostate tissue (BPH) in the nude mouse. BPH fragments 
      prepared from fresh surgical specimens were implanted subcutaneously (s.c.), 
      intraperitoneally (i.p.), or under the renal capsules (r.c.) into male Beige nude 
      mice, which had been implanted s.c. with a Silastic tube filled with 
      4-dihydrotestosterone (DHT) or cholesterol. Two weeks later the BPH tissues were 
      removed from the mouse and examined microscopically. The implants from all three 
      sites maintained a comparable morphology, with epithelial and/or 
      angio-leiomyomatous stromal hyperplastic appearance, without striking signs of 
      atrophy, irrespective of supplementation with DHT. Expression of proliferating 
      cell nuclear antigen in the implants was comparable, indicating that there was no 
      significant influence of implantation site on the proliferative ability of either 
      epithelia or the stromal fibroblasts. The PCNA-positive cells in the implants, 
      including the vascular and myofibrous elements, hybridized in situ to a 
      human-specific repeated-sequence DNA probe, indicating that these proliferating 
      cells were of human origin. Our data suggest that during the early phases of the 
      adaptation and maintenance of BPH implants, survival of epithelial cells is 
      actively supported by fibro-vascular mesenchymal elements of the prostate grafts 
      in a manner apparently unaffected by DHT supplements.
FAU - Soos, G
AU  - Soos G
AD  - Michigan Cancer Foundation, Detroit 48201-1379, USA.
FAU - Debiec-Rychter, M
AU  - Debiec-Rychter M
FAU - Jones, R F
AU  - Jones RF
FAU - Zukowski, K
AU  - Zukowski K
FAU - Haas, G P
AU  - Haas GP
FAU - Wang, C Y
AU  - Wang CY
LA  - eng
GR  - CA23800/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 08J2K08A3Y (Dihydrotestosterone)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Dihydrotestosterone/pharmacology
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Prostate/*transplantation
MH  - Prostatic Hyperplasia/*pathology
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1995 Sep-Oct;15(5B):2007-11.