PMID- 8574641
OWN - NLM
STAT- MEDLINE
DCOM- 19960312
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 695
IP  - 1
DP  - 1995 Oct 9
TI  - Release of serotonin induced by 3,4-methylenedioxymethamphetamine (MDMA) and 
      other substituted amphetamines in cultured fetal raphe neurons: further evidence 
      for calcium-independent mechanisms of release.
PG  - 10-8
AB  - The substituted amphetamines 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyamphetamine (MDA), p-chloro-amphetamine (PCA) and fenfluramine 
      (FEN) all exert their effects by releasing serotonin (5-HT) from presynaptic 
      nerve terminals. In the current study, we examined the ability of these agents to 
      induce the release of 5-HT in culture fetal raphe neurons. The data indicate that 
      the rank order of release potencies for these agents was 
      (+/-)PCA>(+)MDMA=(+)MDA=(+/-)FEN. Studies examining the role fo calcium in 5-HT 
      release demonstrate that preventing calcium influx with L- and N-type calcium 
      channel blockers inhibits potassium-stimulated release of -3H-5-HT but has no 
      effect on release induced by the substituted amphetamines. Furthermore, omitting 
      calcium from the extracellular media or depleting the vesicular pool of 
      neurotransmitter with continual potassium stimulation did not affect the release 
      of -3H-5-HT induced by these compounds. Administration of fluoxetine prior to the 
      substituted amphetamines significantly attenuated the releasing effects of these 
      agents, while producing no effect on potassium-stimulated release. These results 
      are consistent with the notion that the amphetamines induce release of 
      cytoplasmic 5-HT via the plasma membrane transporter.
FAU - Wichems, C H
AU  - Wichems CH
AD  - Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake 
      Forest University, Winston-Salem, NC 27157-1083, USA.
FAU - Hollingsworth, C K
AU  - Hollingsworth CK
FAU - Bennett, B A
AU  - Bennett BA
LA  - eng
GR  - NIDA 05073/DA/NIDA NIH HHS/United States
GR  - NIDA 06634/DA/NIDA NIH HHS/United States
GR  - NIDA 07246/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 2DS058H2CF (Fenfluramine)
RN  - 333DO1RDJY (Serotonin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - CK833KGX7E (Amphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amphetamine/*pharmacology
MH  - Animals
MH  - Calcium/*pharmacology
MH  - Cells, Cultured/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Fenfluramine/pharmacology
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Potassium Chloride/pharmacology
MH  - Raphe Nuclei/*drug effects/metabolism
MH  - Rats
MH  - Serotonin/*metabolism
EDAT- 1995/10/09 00:00
MHDA- 1995/10/09 00:01
CRDT- 1995/10/09 00:00
PHST- 1995/10/09 00:00 [pubmed]
PHST- 1995/10/09 00:01 [medline]
PHST- 1995/10/09 00:00 [entrez]
AID - 0006-8993(95)00774-K [pii]
AID - 10.1016/0006-8993(95)00774-k [doi]
PST - ppublish
SO  - Brain Res. 1995 Oct 9;695(1):10-8. doi: 10.1016/0006-8993(95)00774-k.